Validation Data Gallery
|Positive WB detected in||mouse thymus tissue, rat spleen tissue, human lymph node tissue|
|Positive IHC detected in||human tonsillitis tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||PMA and ionomycin treated MOLT-4 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66220-1-Ig targets PD-1/CD279 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.
|Tested Reactivity||human, rat, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||PD-1/CD279 fusion protein Ag12470|
|Full Name||programmed cell death 1|
|Calculated molecular weight||288 aa, 32 kDa|
|Observed molecular weight||32 kDa, 47-55 kDa|
|GenBank accession number||BC074740|
|Gene ID (NCBI)||5133|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).
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CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wiesława (Verified Customer) (12-08-2022)
works very well
Macarena Lucia (Verified Customer) (10-17-2022)
Mona (Verified Customer) (10-17-2021)
Staining of paraffin-embedded lung tissue from hamster. Antigen retrieval was performed with Tris-EDTA (pH 9)