PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4H4D1

Cat no : 66220-1-Ig

Synonyms

CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



Tested Applications

Positive WB detected inmouse thymus tissue, rat spleen tissue, human lymph node tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inPMA and ionomycin treated MOLT-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66220-1-Ig targets PD-1/CD279 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PD-1/CD279 fusion protein Ag12470
Full Name programmed cell death 1
Calculated molecular weight 288 aa, 32 kDa
Observed molecular weight 32 kDa, 47-55 kDa
GenBank accession numberBC074740
Gene symbol PDCD1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

Protocols

Product Specific Protocols
WB protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IHC protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IF protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade.

Authors - Seungwon Yang
mouseWB

Cancers (Basel)

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Authors - Patrick Nasarre
humanWB

Aging (Albany NY)

Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma.

Authors - Jing-Yuan Cao
mouseWB, IF

CNS Neurosci Ther

The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway.

Authors - Yan Shen
mouse,humanIHC,IF

Front Pharmacol

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Authors - Shuhui Zhang
humanIHC

Clin Exp Immunol

CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.

Authors - Sirui Qian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wiesława (Verified Customer) (12-08-2022)

works very well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000 overnight
  • Cell Tissue Type: human cell lines (25 μg): HL-60, NK-92; mouse thymus (25-45 μg)
PD-1/CD279 Antibody Western Blot validation (1:5000 overnight dilution) in human cell lines (25 μg): HL-60, NK-92; mouse thymus (25-45 μg) (Cat no:66220-1-Ig)
FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Mona (Verified Customer) (10-17-2021)

Staining of paraffin-embedded lung tissue from hamster. Antigen retrieval was performed with Tris-EDTA (pH 9)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: hamster (lung)