CD80 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• CD80 Polyclonal antibody (14292-1-AP)

CD80 Polyclonal Antibody for WB, ELISA

Cat No. 14292-1-AP Copied!

Publications (48)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA

Synonyms

Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1, B7-1

• 

  WB analysis using 14292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human peripheral blood leukocyte using 14292-1-AP

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CD80 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: RAW 264.7 cells, human peripheral blood leukocyte, Daudi cells, Raji cells, Ramos cells, J774A.1 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14292-1-AP targets CD80 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5615

  Product name: Recombinant human B7-1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 36-228 aa of BC042665

  Sequence: IHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQT

• Full Name: CD80 molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC042665
• Gene Symbol: CD80
• Gene ID (NCBI): 941
• RRID: AB_10640809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33681
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

What published studies show

The CD80 Polyclonal antibody (catalog no. 14292-1-AP) has 48 citations. These appear in journals including Cell Research, Bioactive Materials, ACS Nano, J Nanobiotechnology, Biomaterials, J Control Release, Small, J Immunother Cancer, Int J Biol Sci, J Transl Med, iScience, PLoS Pathog, and Mol Immunol. Research fields center on macrophage polarization (M1/M2), tumor immunotherapy, photodynamic therapy, inflammation, and disease models such as cancer, atherosclerosis, spinal cord injury, and acute lung injury, with applications mainly in WB and ELISA.

Automatically generated from the 48 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Cell Res; Dynamic magneto-mechanical force in lysosomes induces durable macrophage repolarization for antitumor immunity.; Authors - Yingze Li
mouse		Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
mouse	WB	ACS Nano; Antigen Self-Presented Personalized Nanovaccines Boost the Immunotherapy of Highly Invasive and Metastatic Tumors; Authors - Tingting Wang
mouse		J Nanobiotechnology; Pro-efferocytic macrophage membrane biomimetic nanoparticles for the synergistic treatment of atherosclerosis via competition effect; Authors - Xuan Sha
mouse	WB	J Nanobiotechnology; Curcumin/pEGCG-encapsulated nanoparticles enhance spinal cord injury recovery by regulating CD74 to alleviate oxidative stress and inflammation; Authors - Tianjun Chen
mouse		J Nanobiotechnology; Targeting and repolarizing M2-like tumor-associated macrophage-mediated MR imaging and tumor immunotherapy by biomimetic nanoparticles; Authors - Lijuan Chong