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WB analysis of A549 using 14304-1-AP
WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells.
WB analysis of SH-SY5Y using 14304-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 14304-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14304-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14304-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14304-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14304-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse kidney using 14304-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14304-1-AP (ACK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14304-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14304-1-AP (ACK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

ACK1 Polyclonal antibody

Name: ACK1 Polyclonal antibody
Brand: Proteintech
SKU: 14304-1-AP

ACK1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14304-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TNK2, ACK, ACK 1, ACK-1, Activated CDC42 kinase 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, HepG2 cells, HT-1080 cells, A431 cells, mouse brain tissue, HeLa cells, A549 cells
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

14304-1-AP targets ACK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5419 Product name: Recombinant human ACK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-385 aa of BC028164 Sequence: MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFL Predict reactive species
Full Name	tyrosine kinase, non-receptor, 2
Calculated Molecular Weight	115 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC028164
Gene Symbol	TNK2
Gene ID (NCBI)	10188
RRID	AB_11041713
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07912
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.

Protocols

Product Specific Protocols
IHC protocol for ACK1 antibody 14304-1-AP	Download protocol
WB protocol for ACK1 antibody 14304-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Microbiol The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling. Authors - Lei He View Article
mouse	WB	Biochim Biophys Acta Mol Cell Res Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport Authors - G F Sengül View Article