TOB1 Polyclonal antibody

TOB1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14915-1-AP

Synonyms

APRO6, PIG49, Protein Tob1, TOB, TOB1, Transducer of erbB 2 1, transducer of ERBB2, 1, TROB, TROB1



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14915-1-AP targets TOB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOB1 fusion protein Ag6714
Full Name transducer of ERBB2, 1
Calculated molecular weight 38 kDa
Observed molecular weight 38-45 kDa
GenBank accession numberBC031406
Gene symbol TOB1
Gene ID (NCBI) 10140
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for TOB1 antibody 14915-1-APDownload protocol
IHC protocol for TOB1 antibody 14915-1-APDownload protocol
IF protocol for TOB1 antibody 14915-1-APDownload protocol
IP protocol for TOB1 antibody 14915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Transl Res

Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.

Authors - Dong Wang
humanWB,IHC

Cancer Med

TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.

Authors - Yuru Bai
humanWB

Oncotarget

Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.

Authors - Jin Zhu