|Positive WB detected in||HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14915-1-AP targets TOB1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TOB1 fusion protein Ag6714|
|Full Name||transducer of ERBB2, 1|
|Calculated molecular weight||38 kDa|
|Observed molecular weight||38-45 kDa|
|GenBank accession number||BC031406|
|Gene ID (NCBI)||10140|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.
|Product Specific Protocols|
|WB protocol for TOB1 antibody 14915-1-AP||Download protocol|
|IHC protocol for TOB1 antibody 14915-1-AP||Download protocol|
|IF protocol for TOB1 antibody 14915-1-AP||Download protocol|
|IP protocol for TOB1 antibody 14915-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Am J Transl Res
Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.
TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.
Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.