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TPH1 Polyclonal antibody

TPH1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 20879-1-AP

Synonyms

TPH, TPH1, TPRH, TRPH, Tryptophan 5 hydroxylase 1, Tryptophan 5 monooxygenase 1, tryptophan hydroxylase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using TPH1 Polyclonal antibody (20879-1-AP)

    WB analysis of SH-SY5Y using 20879-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20879-1-AP (TPH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20879-1-AP is TPH1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPH1 fusion protein Ag14978
Full Name tryptophan hydroxylase 1
Calculated Molecular Weight 466 aa, 53 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC106739
Gene Symbol TPH1
Gene ID (NCBI) 7166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tryptophan hydroxylase 1(TPH1) catalyzes the rate-limiting step in the synthesis of serotonin in the periphery. Recently, it has been shown that expression of the tryptophan hydroxylase 1 gene is increased in lungs and pulmonary endothelial cells from patients with idiopathic pulmonary arterial hypertension. It catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. The protein has low expression.