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TPTE Polyclonal antibody

TPTE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12982-1-AP

Synonyms

Cancer/testis antigen 44, CT44, PTEN2, TPTE, Tumor antigen BJ HCC 5

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of HepG2 using 12982-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of HepG2 using 12982-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of HEK-293 using 12982-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of HEK-293 using 12982-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of human testis using 12982-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of human testis using 12982-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of BxPC-3 using 12982-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of BxPC-3 using 12982-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of mouse testis using 12982-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TPTE Polyclonal antibody (12982-1-AP)

    WB analysis of HepG2 using 12982-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12982-1-AP (TPTE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TPTE Polyclonal antibody (12982-1-AP)

    IHC staining of human testis using 12982-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TPTE Polyclonal antibody (12982-1-AP)

    IHC staining of human testis using 12982-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12982-1-AP (TPTE Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using TPTE Polyclonal antibody (12982-1-AP)

    IF Staining of BxPC-3 using 12982-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 12982-1-AP (TPTE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, human testis tissue, BxPC-3 cells, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12982-1-AP targets TPTE in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPTE fusion protein Ag3606
Full Name transmembrane phosphatase with tensin homology
Calculated Molecular Weight 62 kDa, 64 kDa
Observed Molecular Weight 62 kDa, 64 kDa
GenBank Accession NumberBC028719
Gene Symbol TPTE
Gene ID (NCBI) 7179
RRIDAB_2206336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TPTE(Transmembrane phosphatase with tensin homology) is also named as CT44, BJ-HCC-5 and belongs to the non receptor class of the protein-tyrosine phosphatase family. The gene encodes a predicted polypeptide of 551 amino acids with at least 2 potential transmembrane domains and a tyrosine phosphatase motif. TPTE may be involved in the signal transduction pathways of the endocrine or spermatogenic function of the testis(PMID:10598804). It is putatively involved in linking the cell membrane to cytoskeleton. This protein is very similar in sequence to PTEN through the phosphatase and C2 domains, but lacks a PDZ binding motif and an extensively phosphorylated C-terminal tail(PMID:17240336). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TPTE antibody 12982-1-APDownload protocol
IHC protocol for TPTE antibody 12982-1-APDownload protocol
IF protocol for TPTE antibody 12982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols