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  • KD/KO Validated

TRAF4 Monoclonal antibody

TRAF4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2C9A11

Cat no : 66755-1-Ig

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, THP-1 cells, U-937 cells, NCCIT cells, human brain tissue, pig brain tissue, HEK-293 cells, Ramos cells, Daudi cells, Raji cells, pig spleen tissue
Positive IHC detected inhuman urothelial carcinoma tissue, human lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66755-1-Ig targets TRAF4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF4 fusion protein Ag27221
Full Name TNF receptor-associated factor 4
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC001769
Gene Symbol TRAF4
Gene ID (NCBI) 9618
RRIDAB_2882101
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.

Protocols

Product Specific Protocols
WB protocol for TRAF4 antibody 66755-1-IgDownload protocol
IHC protocol for TRAF4 antibody 66755-1-IgDownload protocol
IF protocol for TRAF4 antibody 66755-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

TRAF4 Promotes the Proliferation of Glioblastoma by Stabilizing SETDB1 to Activate the AKT Pathway

Authors - Hongyu Gu
  • KD Validated
mouseWB

Anticancer Drugs

High expression of TRAF4 predicts poor prognosis in tamoxifen-treated breast cancer and promotes tamoxifen resistance.

Authors - Jun Zhou
  • KD Validated