CoraLite® Plus 488-conjugated TRAF4 Monoclonal antibody

TRAF4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C9A11

Cat no : CL488-66755

Synonyms

CART1, MLN 62, MLN62, RING finger protein 83, RNF83, TRAF4

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-66755

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66755 (TRAF4 antibody) at dilution of 1:100.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66755 targets TRAF4 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAF4 fusion protein Ag27221
• Full Name: TNF receptor-associated factor 4
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC001769
• Gene Symbol: TRAF4
• Gene ID (NCBI): 9618
• RRID: AB_2883380
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The tumor necrosis factor receptor-associated factor (TRAF) family, consisting of seven members (TRAF1-7), is a group of signaling adaptors which participate in various signaling pathways through binding of the tumor necrosis factor receptor superfamily. As one of the important member of TRAF family proteins, TRAF4 was initially isolated from breast carcinomas and identified as the first member of the TRAF family to be up-regulated in human carcinomas. TRAF4 is required during embryogenesis in key biological processes including the formation of the trachea, the development of the axial skeleton, and the closure of the neural tube. TRAF4 deficiency leads to severe developmental alterations.