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  • KD/KO Validated

KAP1 Antibody

KAP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15202-1-AP

Synonyms

KAP 1, KAP1, KRAB associated protein 1, KRAB interacting protein 1, KRIP 1, Nuclear corepressor KAP 1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1B, TRIM28, tripartite motif containing 28



Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, rat spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15202-1-AP targets KAP1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KAP1 fusion protein Ag7350
Full Name tripartite motif-containing 28
Calculated molecular weight 89 kDa
Observed molecular weight 100 kDa
GenBank accession number BC004978
Gene symbol TRIM28
Gene ID (NCBI) 10155
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

Protocols

Product Specific Protocols
WB protocol for KAP1 antibody 15202-1-APDownload protocol
IHC protocol for KAP1 antibody 15202-1-APDownload protocol
IF protocol for KAP1 antibody 15202-1-APDownload protocol
IP protocol for KAP1 antibody 15202-1-APDownload protocol
FC protocol for KAP1 antibody 15202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Elife

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

Authors - Xiancai Ma
mouseWB

DNA Repair (Amst)

ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress.

Authors - Rui Liu
humanWB, IHC

Cancer Epidemiol

TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.

Authors - Liu Lei L
  • KD Validated
humanWB

Oncol Rep

PMID: 28498400

Authors - Lei Liu
mouseWB, IHC

Hepatology

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Authors - Bojkowska Karolina K
  • KO Validated