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KAP1 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 15202-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

KAP1 Antibody WB HeLa cells 15202-1-AP

WB result of KAP1 antibody (15202-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KAP1 transfected HeLa cells

KAP1 Antibody WB HeLa cells 15202-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP(KAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

KAP1 Antibody IHC human colon cancer tissue 15202-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer using 15202-1-AP(KAP1 antibody) at dilution of 1:100 (under 10x lens)

KAP1 Antibody IHC human colon cancer tissue 15202-1-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer using 15202-1-AP(KAP1 antibody) at dilution of 1:100 (under 40x lens)

KAP1 Antibody IF Hela cells 15202-1-AP

Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KAP1 Antibody IP HeLa cells 15202-1-AP

IP Result of anti-KAP1 (IP:15202-1-AP, 4ug; Detection:15202-1-AP 1:1000) with HeLa cells lysate 1200ug.

KAP1 Antibody FC HeLa cells 15202-1-AP

1X10^6 HeLa cells were stained with 0.2ug KAP1 antibody (15202-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, mouse testis tissue, rat spleen tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
KAP1 fusion protein Ag7350
Full name:
tripartite motif-containing 28
Calculated molecular weight:
89 kDa
Observed molecular weight:
100 kDa
GenBank accession number:
BC004978
Gene ID (NCBI):
10155
Gene symbol
TRIM28
Synonyms
KAP 1, KAP1, KRAB associated protein 1, KRAB interacting protein 1, KRIP 1, Nuclear corepressor KAP 1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1B, TRIM28, tripartite motif containing 28

Background

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. （PMID: 18590578 ）


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