- Featured Product
- KD/KO Validated
KAP1 Polyclonal antibody
KAP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, chIP, ELISA
Conjugate
Unconjugated
Cat no : 15202-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, rat spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
15202-1-AP targets KAP1 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KAP1 fusion protein Ag7350 |
| Full Name | tripartite motif-containing 28 |
| Calculated molecular weight | 89 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC004978 |
| Gene symbol | TRIM28 |
| Gene ID (NCBI) | 10155 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. （PMID: 18590578 ）
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KAP1 antibody 15202-1-AP | Download protocol |
| IHC protocol for KAP1 antibody 15202-1-AP | Download protocol |
| IF protocol for KAP1 antibody 15202-1-AP | Download protocol |
| IP protocol for KAP1 antibody 15202-1-AP | Download protocol |
| FC protocol for KAP1 antibody 15202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
DNA Repair (Amst) ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress. | ||
Cancer Epidemiol TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.
| ||
Proc Natl Acad Sci U S A TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks. | ||
Hepatology Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.
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