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  • Featured Product
  • KD/KO Validated

TRIM38 Polyclonal antibody

TRIM38 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13405-1-AP

Synonyms

RING finger protein 15, RNF15, RORET, TRIM38, tripartite motif containing 38, Zinc finger protein RoRet

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    WB analysis of human liver using 13405-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    WB analysis of human colon using 13405-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TRIM38 Polyclonal antibody (13405-1-AP)

    WB analysis of HEK-293 using 13405-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13405-1-AP (TRIM38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human liver using 13405-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human liver using 13405-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human lung cancer using 13405-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TRIM38 Polyclonal antibody (13405-1-AP)

    IHC staining of human lung cancer using 13405-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13405-1-AP (TRIM38 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using TRIM38 Polyclonal antibody (13405-1-AP)

    IF Staining of L02 using 13405-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed L02 cells using 13405-1-AP (TRIM38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue, HEK-293 cells, human colon tissue
Positive IHC detected inhuman liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13405-1-AP targets TRIM38 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM38 fusion protein Ag3840
Full Name tripartite motif-containing 38
Calculated Molecular Weight 465 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC026930
Gene Symbol TRIM38
Gene ID (NCBI) 10475
RRIDAB_2208307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proinflammatory cytokines TNFα and IL-1β play central roles in many diseases, including infectious diseases, autoimmunity, and cancers. TRIM38 belongs to the TRIM protein family that is involved in various biological processes, including cell proliferation and apoptosis, innate immunity, and inflammatory response. It has been shown that TRIM38 acts as a suppressor in TOLL-like receptor (TLR)-mediated IFN-β induction by promoting degradation of TRAF6 and NAP1 through the ubiquitinproteasome system. (PMID:24434549)

Protocols

Product Specific Protocols
WB protocol for TRIM38 antibody 13405-1-APDownload protocol
IHC protocol for TRIM38 antibody 13405-1-APDownload protocol
IF protocol for TRIM38 antibody 13405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

Tertiary lymphoid structure stratifies glioma into three distinct tumor subtypes.

Authors - Xingwang Zhou
View Article
mouseWB

Int Immunopharmacol

TRIM38 protects chondrocytes from IL-1β-induced apoptosis and degeneration via negatively modulating nuclear factor (NF)-κB signaling.

Authors - Shouye Hu
  • KD Validated
View Article
mouseWB,IHC

J Lipid Res

Tripartite motif 38 alleviates the pathological process of NAFLD/NASH by promoting TAB2 degradation

Authors - Xinxin Yao
View Article