Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11224-1-AP

Synonyms

alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, TUBA1B, tubulin, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, tubulin, alpha 1b, α-tubulin


Filter:
  • Western Blot (WB) analysis of various lysates using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    WB analysis using 11224-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11224-1-AP (alpha Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IP experiment of HeLa using 11224-1-AP

    IP result of anti-Alpha Tubulin (IP:11224-1-AP, 3ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 5000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IHC staining of human colon cancer using 11224-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11224-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IHC staining of human colon cancer using 11224-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11224-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IHC staining of human liver cancer using 11224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IHC staining of human endometrial cancer using 11224-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of Lymphatic endothelial cells cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IF Staining of Lymphatic endothelial cells using 11224-1-AP

    Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IF Staining of HeLa using 11224-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Tubulin antibody (11224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IF Staining of MCF-7 using 11224-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IF Staining of HepG2 using 11224-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    IF Staining of HepG2 using 11224-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11224-1-AP (alpha Tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using Alpha Tubulin Polyclonal antibody (11224-1-AP)

    FC experiment of HEK-293 using 11224-1-AP

    1X10^6 HEK-293 cells were stained with 0.2ug alpha Tubulin antibody (11224-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

"Alpha Tubulin Antibodies" Comparison

View side-by-side comparison of Alpha Tubulin antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, rat brain
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells, MCF-7 cells, Lymphatic endothelial cells cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11224-1-AP targets Alpha Tubulin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, monkey, zebrafish, P. lividus, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha Tubulin fusion protein Ag1727
Full Name tubulin, alpha 1b
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC009314
Gene Symbol TUBA1B
Gene ID (NCBI) 10376
RRIDAB_2210206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the function of alpha tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.

 Where is alpha-tubulin expressed?

Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton. 

What are the post-translational modifications of alpha tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3  Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

 1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).

3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).


Protocols

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IF protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
IP protocol for Alpha Tubulin antibody 11224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
humanWB

Cell Res

SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses

Authors - Shufen Li
humanWB

Nature

Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity.

Authors - Kuan-Chuan Pao
humanWB

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng
humanWB

Nat Med

Therapeutic efficacy of antisense oligonucleotides in mouse models of CLN3 Batten disease.

Authors - Jessica L Centa
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kamal (Verified Customer) (02-15-2024)

One of the best antibody to detect alpha-tubulin. Works well when diluted in 1X TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Mouse liver tissue
FH

Brogan (Verified Customer) (11-09-2022)

Used as a control for C elegans western blot

  • Applications: Western Blot
FH

WEI (Verified Customer) (03-03-2022)

Good antibody for detect tubulin proteins

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:8000
  • Cell Tissue Type: Mouse Heart
FH

Maria (Verified Customer) (06-19-2021)

a Tubulin in human primary fibroblasts. 10 ug of total protein with DTT. 4-15 TGX gel. 3% BSA in PBST 1h RT. 1:1000 primary ab in BSA 3% in PBST (0.1% Tween) O/N 4ºC. Secondary ab 1:5000 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human primary fibroblasts
Alpha Tubulin Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:11224-1-AP)
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed SH-SY5Y cells by Alpha Tubulin Polyclonal antibody at dilution of 1:100 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y cells
FH

Chao (Verified Customer) (03-12-2020)

Good sharp clear band in western blot.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

Wei (Verified Customer) (01-30-2020)

Very good, sharp band for internal control.The protein is better than GAPDH for quantification in heart diseases.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse Heart
FH

KARTHIKEYAN (Verified Customer) (11-20-2019)

I used this antibody in mouse brain endothelial cell line, it worked really well, i would definitely recommend this product.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain Endothelial cell line
Alpha Tubulin Antibody Western Blot, validation (1:1000 dilution) in Brain Endothelial cell line (Cat no:11224-1-AP)
FH

Pooja (Verified Customer) (11-05-2019)

This antibody works very well as loading control. Even lower dilution can be use.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MCF7 cells
FH

Yuan (Verified Customer) (11-02-2019)

Very nice staining for A549 cells with 1:200 dilution

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: A549 cell
Alpha Tubulin Antibody Immunofluorescence, validation (1:200 dilution) in A549 cell (Cat no:11224-1-AP)
FH

Kishor (Verified Customer) (01-30-2019)

I got good results for Western Blotting (1:1000) and IF (1:300)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
Alpha Tubulin Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:11224-1-AP)
FH

kk (Verified Customer) (11-21-2018)

Excellent product, single and sharp band in all my blots

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: primary mouse islets