Validation Data Gallery
|Positive WB detected in||A549 cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, HepG2 cells, Lymphatic endothelial cells cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:15000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11224-1-AP targets Alpha Tubulin in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, hamster, human, monkey, mouse, pig, rat, Tribolium castaneum, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha Tubulin fusion protein Ag1727|
|Full Name||tubulin, alpha 1b|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC009314|
|Gene ID (NCBI)||10376|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
What is the function of alpha tubulin?
Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerises to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport and are also involved in cell division.
Where is alpha-tubulin expressed?
Alpha tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.
What are the post-translational modifications of alpha tubulin?
The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerised mictotubule. For example, the first modification to be identified was detyrosination1, as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyronisation along the structure, regulating protein interactions and allowing subcellular compartments to be defined.2,3 Polyglutamylation also occurs on several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).
1. Gundersen, G. G., Khawaja, S. & Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105, 251-64 (1987).
2. Galjart, N. Plus-End-Tracking Proteins and Their Interactions at Microtubule Ends. Curr. Biol. 20, R528-R537 (2010).
3. Jiang, K. & Akhmanova, A. Microtubule tip-interacting proteins: a view from both ends. Curr. Opin. Cell Biol. 23, 94-101 (2011).
|Product Specific Protocols|
|WB protocol for Alpha Tubulin antibody 11224-1-AP||Download protocol|
|IHC protocol for Alpha Tubulin antibody 11224-1-AP||Download protocol|
|IF protocol for Alpha Tubulin antibody 11224-1-AP||Download protocol|
|IP protocol for Alpha Tubulin antibody 11224-1-AP||Download protocol|
|FC protocol for Alpha Tubulin antibody 11224-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Chao (Verified Customer) (03-12-2020)
Good sharp clear band in western blot.
Wei (Verified Customer) (01-30-2020)
Very good, sharp band for internal control.The protein is better than GAPDH for quantification in heart diseases.
KARTHIKEYAN (Verified Customer) (11-20-2019)
I used this antibody in mouse brain endothelial cell line, it worked really well, i would definitely recommend this product.
Pooja (Verified Customer) (11-05-2019)
This antibody works very well as loading control. Even lower dilution can be use.
Yuan (Verified Customer) (11-02-2019)
Very nice staining for A549 cells with 1:200 dilution
Kishor (Verified Customer) (01-30-2019)
I got good results for Western Blotting (1:1000) and IF (1:300)
kk (Verified Customer) (11-21-2018)
Excellent product, single and sharp band in all my blots