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UBE2E2 Antibody

UBE2E2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11844-1-AP

Synonyms

UBCH8, UBE2E2, Ubiquitin carrier protein E2, Ubiquitin protein ligase E2



Tested Applications

Positive WB detected inhuman thyroid gland tissue, human brain tissue, mouse embryo tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11844-1-AP targets UBE2E2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2E2 fusion protein Ag2509
Full Name ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
Calculated molecular weight 201aa,22 kDa
Observed molecular weight 22 kDa
GenBank accession number BC022332
Gene symbol UBE2E2
Gene ID (NCBI) 7325
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).

Protocols

Product Specific Protocols
WB protocol for UBE2E2 antibody 11844-1-APDownload protocol
IHC protocol for UBE2E2 antibody 11844-1-APDownload protocol
IF protocol for UBE2E2 antibody 11844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

ISG15 inhibits cancer cell growth and promotes apoptosis.

Authors - Mei-Juan Zhou
humanWB

Mol Cell Biol

Selective ubiquitylation of p21 and Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating enzymes via the CRL4Cdt2 ubiquitin ligase complex.

Authors - Shibata Etsuko E
humanWB

Genes Dev

Sequential replication-coupled destruction at G1/S ensures genome stability.

Authors - Kate E Coleman
  • KD Validated