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UBC13 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10243-1-AP

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Con: 33 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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UBC13 Antibody WB mouse thymus tissue 10243-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP(UBC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

UBC13 Antibody IP MCF-7 cells 10243-1-AP

IP Result of anti-UBC13 (IP:10243-1-AP, 4ug; Detection:10243-1-AP 1:600) with MCF-7 cells lysate 1280ug.

UBC13 Antibody IHC human breast cancer tissue 10243-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP( UBC13 antibody at dilution of 1:200 (under 10x lens)

UBC13 Antibody IHC human breast cancer tissue 10243-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP( UBC13 antibody at dilution of 1:200 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
UBC13 fusion protein Ag0292
Full name:
ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Calculated molecular weight:
17 kDa
Observed molecular weight:
17 kDa
GenBank accession number:
BC000396
Gene ID (NCBI):
7334
Gene symbol
UBE2N
Synonyms
BLU, UBC13, UbcH ben, UBE2N, Ubiquitin carrier protein N, Ubiquitin protein ligase N

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair


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