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UBC13 Polyclonal antibody

UBC13 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10243-1-AP

Synonyms

BLU, UBC13, UbcH ben, UBE2N, Ubiquitin carrier protein N, Ubiquitin protein ligase N

Validation Data Gallery

  • WB analysis of mouse thymus using 10243-1-AP

    WB analysis of mouse thymus using 10243-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP (UBC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 10243-1-AP

    IP experiment of MCF-7 using 10243-1-AP

    IP Result of anti-UBC13 (IP:10243-1-AP, 4ug; Detection:10243-1-AP 1:600) with MCF-7 cells lysate 1280ug.

  • IHC staining of human breast cancer using 10243-1-AP

    IHC staining of human breast cancer using 10243-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 10243-1-AP

    IHC staining of human breast cancer using 10243-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP (UBC13 antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10243-1-AP targets UBC13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC13 fusion protein Ag0292
Full Name ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC000396
Gene symbol UBE2N
Gene ID (NCBI) 7334
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair

Protocols

Product Specific Protocols
WB protocol for UBC13 antibody 10243-1-APDownload protocol
IHC protocol for UBC13 antibody 10243-1-APDownload protocol
IP protocol for UBC13 antibody 10243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jessica (Verified Customer) (11-14-2019)

Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.

  • Applications: Western Blot,
  • Cell Tissue Type: 293T cells