Back

UBC13 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10243-1-AP

1 customer review

-_-

Freight/Packing

Con: 33 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IP, IHC, ELISA
Application not tested? Please see our Guarantee


Images See all

UBC13 Antibody WB mouse thymus tissue 10243-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10243-1-AP(UBC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

UBC13 Antibody IP MCF-7 cells 10243-1-AP

IP Result of anti-UBC13 (IP:10243-1-AP, 4ug; Detection:10243-1-AP 1:600) with MCF-7 cells lysate 1280ug.

UBC13 Antibody IHC human breast cancer tissue 10243-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP( UBC13 antibody at dilution of 1:200 (under 10x lens)

UBC13 Antibody IHC human breast cancer tissue 10243-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10243-1-AP( UBC13 antibody at dilution of 1:200 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
UBC13 fusion protein Ag0292
Full name:
ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Calculated molecular weight:
17 kDa
Observed molecular weight:
17 kDa
GenBank accession number:
BC000396
Gene ID (NCBI):
7334
Gene symbol
UBE2N
Synonyms
BLU, UBC13, UbcH ben, UBE2N, Ubiquitin carrier protein N, Ubiquitin protein ligase N

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

UBC13 Rabbit Polyclonal antibody

Jessica (Verified Customer), 11-14-2019
Applications
Western Blot,
No image submitted with review

No image submitted with review

Cell/Tissue Type
293T cells
Lot Number
Antibody works well for detecting the endogenous Ubc13 protein in 293T cells. Strong signal and few non specific bands.

Back
to top