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UBE2R2 Polyclonal antibody

UBE2R2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 14077-1-AP

Synonyms

CDC34B, UBC3B, UBE2R2, Ubiquitin carrier protein R2, Ubiquitin protein ligase R2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using UBE2R2 Polyclonal antibody (14077-1-AP)

    WB analysis of Jurkat using 14077-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using UBE2R2 Polyclonal antibody (14077-1-AP)

    WB analysis of mouse spleen using 14077-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using UBE2R2 Polyclonal antibody (14077-1-AP)

    WB analysis of HeLa using 14077-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14077-1-AP (UBE2R2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using UBE2R2 Polyclonal antibody (14077-1-AP)

    IHC staining of human spleen using 14077-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using UBE2R2 Polyclonal antibody (14077-1-AP)

    IHC staining of human spleen using 14077-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14077-1-AP (UBE2R2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBE2R2 Polyclonal antibody (14077-1-AP)

    IF Staining of HeLa using 14077-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14077-1-AP (UBE2R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse spleen tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

14077-1-AP targets UBE2R2 in WB,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2R2 fusion protein Ag5202
Full Name ubiquitin-conjugating enzyme E2R 2
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC047584
Gene Symbol UBE2R2
Gene ID (NCBI) 54926
RRIDAB_2878010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBE2R2 antibody 14077-1-APDownload protocol
IHC protocol for UBE2R2 antibody 14077-1-APDownload protocol
IF protocol for UBE2R2 antibody 14077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

JCI Insight

PMID: 31292296

Authors - Isabelle K Vila
View Article
humanWB

bioRxiv

Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases

Authors - Jason C Collins
View Article
humanWB

Exp Gerontol

p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6

Authors - Ying Cheng
View Article