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USMG5 Polyclonal antibody

USMG5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17716-1-AP

Synonyms

HCV F transactivated protein 2, HCVFTP2, USMG5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USMG5 Polyclonal antibody (17716-1-AP)

    WB analysis using 17716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using USMG5 Polyclonal antibody (17716-1-AP)

    WB analysis of human liver using 17716-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using USMG5 Polyclonal antibody (17716-1-AP)

    WB analysis of human brain using 17716-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of mouse brain using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of mouse brain using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of human placenta using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using USMG5 Polyclonal antibody (17716-1-AP)

    IHC staining of human placenta using 17716-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human liver tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17716-1-AP targets USMG5 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USMG5 fusion protein Ag12086
Full Name up-regulated during skeletal muscle growth 5 homolog (mouse)
Calculated Molecular Weight 58 aa, 7 kDa
Observed Molecular Weight 6 kDa
GenBank Accession NumberBC072683
Gene Symbol USMG5
Gene ID (NCBI) 84833
RRIDAB_2214288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for USMG5 antibody 17716-1-APDownload protocol
IHC protocol for USMG5 antibody 17716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice.

Authors - James B Papizan
View Article
mouseIF

Sci Rep

miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy.

Authors - M Sanson
View Article