VAMP1 Polyclonal antibody

VAMP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13115-1-AP

Synonyms

SYB1, Synaptobrevin 1, VAMP 1, VAMP1



Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13115-1-AP targets VAMP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAMP1 fusion protein Ag3787
Full Name vesicle-associated membrane protein 1 (synaptobrevin 1)
Calculated Molecular Weight 117 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC023286
Gene Symbol VAMP1
Gene ID (NCBI) 6843
RRIDAB_2214772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP1, also named as synaptobrevin 1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP1 is a part of the SNARE (soluble NSF-attachment protein receptor) complex. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP1 is involved in synaptic vesicle exocytosis, a fundamental step in neurotransmitter release.

Protocols

Product Specific Protocols
WB protocol for VAMP1 antibody 13115-1-APDownload protocol
IHC protocol for VAMP1 antibody 13115-1-APDownload protocol
IF protocol for VAMP1 antibody 13115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Pharmacol Res

The role of circTmeff-1 in incubation of context-induced morphine craving.

Authors - Hailei Yu
mouseIF

Eur J Immunol

ACKR1 favors transcellular over paracellular T-cell diapedesis across the blood-brain barrier in neuroinflammation in vitro.

Authors - Luca Marchetti
ratWB

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
mouseIF

EMBO J

Binary organization of epidermal basal domains highlights robustness to environmental exposure

Authors - Sangeeta Ghuwalewala
mouseWB

Neurotoxicology

Heterozygous disruption of beclin 1 alleviates neurotoxicity induced by sub-chronic exposure of arsenite in mice

Authors - Danyang Li