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VDAC1/2 Polyclonal antibody
VDAC1/2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10866-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, 4T1 cells, DU 145 cells, HeLa cells, HL-60 cells, human heart tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, MCF-7 cells, MDA-MB-453s cells, rat heart tissue, rat kidney tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 60 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Product Information
10866-1-AP targets VDAC1/2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, Pieris rapae, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VDAC1/2 fusion protein Ag1144 |
| Full Name | voltage-dependent anion channel 1 |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC008482 |
| Gene symbol | VDAC1 |
| Gene ID (NCBI) | 7416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
| IHC protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
| IF protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
| IP protocol for VDAC1/2 antibody 10866-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine Targeting glutaminase 1 attenuates stemness properties in hepatocellular carcinoma by increasing reactive oxygen species and suppressing Wnt/beta-catenin pathway. | ||
Hum Mol Genet Contribution of mitochondrial ND1 3394T>C mutation to the phenotypic manifestation of Leber's hereditary optic neuropathy. | ||
Theranostics p66Shc Contributes to Liver Fibrosis through the Regulation of Mitochondrial Reactive Oxygen Species. | ||
Nucleic Acids Res Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism. | ||
J Immunol The Otubain YOD1 Suppresses Aggregation and Activation of the Signaling Adaptor MAVS through Lys63-Linked Deubiquitination. | ||
Cell Cycle A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alberto (Verified Customer) (01-09-2020) | Clear sharp band at the expected molecular weight.
 |
FH Wanxia (Verified Customer) (10-22-2019) | Excellent WB results and very low background.
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