|Positive WB detected in||HEK-293 cells, 4T1 cells, DU 145 cells, HeLa cells, HL-60 cells, human heart tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, MCF-7 cells, MDA-MB-453s cells, rat heart tissue, rat kidney tissue|
|Positive IP detected in||mouse skeletal muscle tissue|
|Positive IHC detected in||human colon tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10866-1-AP targets VDAC1/2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Pieris rapae, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VDAC1/2 fusion protein Ag1144|
|Full Name||voltage-dependent anion channel 1|
|Calculated molecular weight||31 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC008482|
|Gene ID (NCBI)||7416|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This polyclonal antibody raised against full-length human VDAC1 protein can cross react with VDAC2.
|Product Specific Protocols|
|WB protocol for VDAC1/2 antibody 10866-1-AP||Download protocol|
|IHC protocol for VDAC1/2 antibody 10866-1-AP||Download protocol|
|IF protocol for VDAC1/2 antibody 10866-1-AP||Download protocol|
|IP protocol for VDAC1/2 antibody 10866-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Alberto (Verified Customer) (01-09-2020)
Clear sharp band at the expected molecular weight.
Wanxia (Verified Customer) (10-22-2019)
Excellent WB results and very low background.