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  • KD/KO Validated

VPRBP Polyclonal antibody

VPRBP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11612-1-AP

Synonyms

DCAF1, HIV 1 Vpr binding protein, KIAA0800, Protein VPRBP, RIP, Vpr (HIV 1) binding protein, Vpr interacting protein, VPRBP



Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells, mouse testis tissue, HeLa cells, K-562 cells, HEK-293 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11612-1-AP targets VPRBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPRBP fusion protein Ag2184
Full Name Vpr (HIV-1) binding protein
Calculated molecular weight 1506 aa, 169 kDa
Observed molecular weight 169 kDa
GenBank accession numberBC022792
Gene symbol VPRBP
Gene ID (NCBI) 9730
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Protocols

Product Specific Protocols
WB protocol for VPRBP antibody 11612-1-APDownload protocol
IHC protocol for VPRBP antibody 11612-1-APDownload protocol
IF protocol for VPRBP antibody 11612-1-APDownload protocol
IP protocol for VPRBP antibody 11612-1-APDownload protocol
FC protocol for VPRBP antibody 11612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virology

HIV-2 viral protein X (Vpx) ubiquitination is dispensable for ubiquitin ligase interaction and effects on macrophage infection.

Authors - McCulley Anna A
humanWB

PLoS One

Defining the Interactions and Role of DCAF1/VPRBP in the DDB1-Cullin4A E3 Ubiquitin Ligase Complex Engaged by HIV-1 Vpr to Induce a G2 Cell Cycle Arrest.

Authors - Francine C A Gérard
humanWB

PLoS One

Differential effects of Vpr on single-cycle and spreading HIV-1 infections in CD4+ T-cells and dendritic cells.

Authors - de Silva Suresh S
  • KD Validated
humanWB

J Biol Chem

HIV-1 Vpr hijacks EDD-DYRK2-DDB1DCAF1 to disrupt centrosome homeostasis.

Authors - Delowar Hossain
  • KD Validated
humanIHC

Cancer Cytopathol

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Authors - Yi-Zhen Liu
mouseWB

J Immunol

VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.

Authors - N Max Schabla
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nikhil (Verified Customer) (09-17-2019)

Very good for both IP and western blot.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Colon cancer cells
VPRBP Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in Colon cancer cells (Cat no:11612-1-AP)