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VPRBP Antibody 27 Publications

Rabbit Polyclonal| Catalog number: 11612-1-AP

Featured Product KD/KO validated

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Con: 25 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

VPRBP Antibody WB HEK-293 cells 11612-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP(VPRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

VPRBP Antibody IHC human prostate cancer tissue 11612-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP( VPRBP Antibody) at dilution of 1:50 (under 10x lens)

VPRBP Antibody IHC human prostate cancer tissue 11612-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP( VPRBP Antibody) at dilution of 1:50 (under 40x lens)

VPRBP Antibody IP HeLa cells 11612-1-AP

IP Result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.

VPRBP Antibody IF HeLa cells 11612-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 11612-1-AP (VPRBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

VPRBP Antibody FC HeLa cells 11612-1-AP

1X10^6 HeLa cells were stained with 0.2ug VPRBP antibody (11612-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, dog
Positive WB detected in:
HEK-293 cells, mouse testis tissue, HeLa cells, PC-3 cells, K-562 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
VPRBP fusion protein Ag2184
Full name:
Vpr (HIV-1) binding protein
Calculated molecular weight:
1506aa,169 kDa
Observed molecular weight:
169 kDa
GenBank accession number:
BC022792
Gene ID (NCBI):
9730
Gene symbol
VPRBP
Synonyms
DCAF1, HIV 1 Vpr binding protein, KIAA0800, Protein VPRBP, RIP, Vpr (HIV 1) binding protein, Vpr interacting protein, VPRBP

Background

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).


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