VPRBP Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 11612-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 25 μg/150 μl

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Species specificity:
human, mouse, rat, dog

Positive WB detected in:
HEK-293 cells,mouse testis tissue, HeLa cells, PC-3 cells, K-562 cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human prostate cancer tissue

Positive IF detected in:
HeLa cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
Vpr (HIV-1) binding protein

Calculated molecular weight:
1506aa,169 kDa

Observed molecular weight:
169 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
VPRBP

Synonyms
DCAF1, HIV 1 Vpr binding protein, KIAA0800, Protein VPRBP, RIP, Vpr (HIV 1) binding protein, Vpr interacting protein, VPRBP
Background

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).


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