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VPREB3 Polyclonal antibody

VPREB3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11915-1-AP

Synonyms

8HS20, N27C7 2, pre B lymphocyte 3, Pre B lymphocyte protein 3, Protein VPreB3, VPREB3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPREB3 Polyclonal antibody (11915-1-AP)

    WB analysis using 11915-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11915-1-AP (VPREB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11915-1-AP targets VPREB3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPREB3 fusion protein Ag2528
Full Name pre-B lymphocyte 3
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC020666
Gene Symbol VPREB3
Gene ID (NCBI) 29802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPREB3 (formerly named 8HS20) is a protein that associates with mu chains in the endoplasmic reticulum of pre-B cell lines (PMID: 8491176; 22684248). VPREB3 is normally expressed by precursor B cells in bone marrow and by a subset of normal germinal center B cells in secondary lymphoid organs. It has been reported that VPREB3 expression is associated with B-cell tumors with c-MYC abnormalities (PMID: 20823132).

Protocols

Product Specific Protocols
WB protocol for VPREB3 antibody 11915-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols