• Featured Product
  • KD/KO Validated

YTHDC1 Polyclonal antibody

YTHDC1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14392-1-AP

Synonyms

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse tissue
Positive IHC detected inmouse brain tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14392-1-AP targets YTHDC1 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDC1 fusion protein Ag5882
Full Name YTH domain containing 1
Calculated Molecular Weight 85 kDa
Observed Molecular Weight~100 kDa
GenBank Accession NumberBC053863
Gene Symbol YTHDC1
Gene ID (NCBI) 91746
RRIDAB_2878052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDC1, also named as KIAA1966 or YT521, is a 727 animo acid protein, which contains 1 YTH domain. YTHDC1 localizes in the nucleus and Interacts with EMD, KHDRBS1/SAM68, KHDRBS3, TRA2B/SFRS10, SC35 and SF2. YTHDC1 as a RNA-binding protein that regulates alternative splice site selection. The calcualted molecular weight of YTHDC1 is 85 kDa, but modified TFEB is about 110 kDa (PMID: 10973987 ).

Protocols

Product Specific Protocols
WB protocol for YTHDC1 antibody 14392-1-APDownload protocol
IHC protocol for YTHDC1 antibody 14392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
humanWB

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen
humanWB,IF

Nucleic Acids Res

N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.

Authors - Haojie Hao
humanWB

Cell Death Differ

YTHDC1-mediated augmentation of miR-30d in repressing pancreatic tumorigenesis via attenuation of RUNX1-induced transcriptional activation of Warburg effect.

Authors - Yichao Hou
mouseRIP

Dev Cell

N6-Adenosine Methylation of Socs1 mRNA Is Required to Sustain the Negative Feedback Control of Macrophage Activation.

Authors - Jie Du
humanWB,IP

Mol Ther Nucleic Acids

METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination.

Authors - Danyang Shen
  • KD Validated