Zyxin Antibody

Zyxin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4D7E7

Cat no : 60254-1-Ig

Synonyms

ESP 2, HED 2, ZYX, zyxin, Zyxin 2



Tested Applications

Positive WB detected inHeLa cells, A431 cells, MCF-7 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue, human cervical cancer tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The 60254-1-Ig immunogen is Fusion Protein Zyxin protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Zyxin fusion protein Ag0419
Full Name zyxin
Calculated molecular weight 80 kDa
Observed molecular weight 78 kDa
GenBank accession number BC008743
Gene symbol ZYX
Gene ID (NCBI) 7791
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.

Protocols

Product Specific Protocols
WB protocol for Zyxin antibody 60254-1-IgDownload protocol
IHC protocol for Zyxin antibody 60254-1-IgDownload protocol
IF protocol for Zyxin antibody 60254-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mater Sci Mater Med

Surface changes of nanotopography by carbon ion implantation to enhance the biocompatibility of silicone rubber: an in vitro study of the optimum ion fluence and adsorbed protein.

Authors - Xianhui Li