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- KD/KO Validated
p38 MAPK Monoclonal antibody
p38 MAPK Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig and More (1)
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
CloneNo.
1A1C2
Cat no : 66234-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, pig heart tissue, human heart tissue, Jurkat cells, MCF-7 cells, HSC-T6 cells |
| Positive IHC detected in | human lung cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 10 publications below |
| IHC | See 1 publications below |
Product Information
66234-1-Ig targets p38 MAPK in WB, IHC,ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | p38 MAPK fusion protein Ag5797 |
| Full Name | mitogen-activated protein kinase 14 |
| Calculated molecular weight | 360 aa, 41 kDa |
| Observed molecular weight | 38-42 kDa |
| GenBank accession number | BC031574 |
| Gene symbol | MAPK14 |
| Gene ID (NCBI) | 1432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p38 MAPK antibody 66234-1-Ig | Download protocol |
| IHC protocol for p38 MAPK antibody 66234-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Med Effects of RhoC downregulation on the angiogenesis characteristics of myeloma vascular endothelial cells. | ||
Toxicol Lett Melamine causes testicular toxicity by destroying blood-testis barrier in piglets. | ||
Free Radic Biol Med Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling. | ||
Mol Med Rep Mda‑7/IL‑24 induces the differentiation of B cell lymphoma via activation of the P38 mitogen activated protein kinase signaling pathway. | ||
Exp Ther Med Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma. |
