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p38 MAPK Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66234-1-Ig |CloneNo.: 1A1C2

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Con: 200 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

p38 MAPK Antibody WB HEK-293 cells 66234-1-Ig

WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells

p38 MAPK Antibody WB human heart tissue 66234-1-Ig

human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig( p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

p38 MAPK Antibody WB HeLa cells 66234-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

p38 MAPK Antibody IHC human lung cancer tissue 66234-1-Ig

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

p38 MAPK Antibody IHC human lung cancer tissue 66234-1-Ig

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, pig, mouse, piglet
Positive WB detected in:
HeLa cells, HEK-293 cells, pig heart tissue, human heart tissue, Jurkat cells, MCF-7 cells, HSC-T6 cells
Positive IHC detected in:
human lung cancer tissue, human gliomas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:6000
IHC : 1:250-1:1000

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
p38 MAPK fusion protein Ag5797
Full name:
mitogen-activated protein kinase 14
Calculated molecular weight:
360aa,41 kDa
Observed molecular weight:
38-42 kDa
GenBank accession number:
BC031574
Gene ID (NCBI):
1432
Gene symbol
MAPK14
Synonyms
CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38 MAPK, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

Background

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.


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