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p38 MAPK Antibody

p38 MAPK Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1C2

Cat no : 66234-1-Ig

Synonyms

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38 MAPK, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, pig heart tissue, human heart tissue, Jurkat cells, MCF-7 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

66234-1-Ig targets p38 MAPK in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse, piglet
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen p38 MAPK fusion protein Ag5797
Full Name mitogen-activated protein kinase 14
Calculated molecular weight 360aa,41 kDa
Observed molecular weight 38-42 kDa
GenBank accession number BC031574
Gene symbol MAPK14
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for p38 MAPK antibody 66234-1-IgDownload protocol
IHC protocol for p38 MAPK antibody 66234-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Cathepsin C promotes microglia M1 polarization and aggravates neuroinflammation via activation of Ca2+-dependent PKC/p38MAPK/NF-κB pathway.

Authors - Qing Liu
humanWB

Cancer Med

Effects of RhoC downregulation on the angiogenesis characteristics of myeloma vascular endothelial cells.

Authors - Zhihua Zhao
pigletWB

Toxicol Lett

Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.

Authors - Lingling Chang
mouseWB

Free Radic Biol Med

Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling.

Authors - Weiche Wu

Mol Med Rep

Mda‑7/IL‑24 induces the differentiation of B cell lymphoma via activation of the P38 mitogen activated protein kinase signaling pathway.

Authors - Ming Ma