- Featured Product
- KD/KO Validated
p38 MAPK Antibody
p38 MAPK Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1A1C2
Cat no : 66234-1-Ig
Synonyms
CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38 MAPK, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, pig heart tissue, human heart tissue, Jurkat cells, MCF-7 cells, HSC-T6 cells |
| Positive IHC detected in | human lung cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 4 publications below |
Product Information
66234-1-Ig targets p38 MAPK in WB, IHC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, piglet |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | p38 MAPK fusion protein Ag5797 |
| Full Name | mitogen-activated protein kinase 14 |
| Calculated molecular weight | 360aa,41 kDa |
| Observed molecular weight | 38-42 kDa |
| GenBank accession number | BC031574 |
| Gene symbol | MAPK14 |
| Gene ID (NCBI) | 1432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cellself-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for p38 MAPK antibody 66234-1-Ig | Download protocol |
| IHC protocol for p38 MAPK antibody 66234-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neuroinflammation Cathepsin C promotes microglia M1 polarization and aggravates neuroinflammation via activation of Ca2+-dependent PKC/p38MAPK/NF-κB pathway. | ||
Cancer Med Effects of RhoC downregulation on the angiogenesis characteristics of myeloma vascular endothelial cells. | ||
Toxicol Lett Melamine causes testicular toxicity by destroying blood-testis barrier in piglets. | ||
Free Radic Biol Med Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling. | ||
Mol Med Rep Mda‑7/IL‑24 induces the differentiation of B cell lymphoma via activation of the P38 mitogen activated protein kinase signaling pathway. |
