• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN alpha 2A protein


Typically ≤ 0.4 ng/mL EC50

Species Reactivity




Cat no : HZ-1066


IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A

Technical Specifications

GeneID 3440
Species Human
Expression HEK293
Activity Typically ≤ 0.4 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01563
Molecular Mass 16 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761).


IFN alpha 2, IFN alpha 2A, IFNA, IFNA2, INFA2, Interferon alpha 2, Interferon alpha A, interferon, alpha 2, LeIF A



ACS Infect Dis

The Nonstructural Protein of Guertu Virus Disrupts Host Defenses by Blocking Antiviral Interferon Induction and Action.

Authors - Yuan-Qin Min

Int J Mol Sci

Interferon-Induced Transmembrane Protein 1 (IFITM1) Promotes Distant Metastasis of Small Cell Lung Cancer.

Authors - Shuichi Sakamoto

Immunol Cell Biol

Regulation of human Th9 differentiation by type I interferons and IL-21.

Authors - Michael TWong

J Virol

Species-specific contribution of the four C-terminal amino acids of influenza A virus NS1 protein to virulence.

Authors - Sébastien MSoubies

Cancer Sci

Combination of tumor necrosis factor-α and epidermal growth factor induces the adrenergic-to-mesenchymal transdifferentiation in SH-SY5Y neuroblastoma cells.

Authors - Yue Huang

Cell Mol Immunol

BCR-ABL-specific CD4+ T-helper cells promote the priming of antigen-specific cytotoxic T cells via dendritic cells.

Authors - NorihiroUeda


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christine (Verified Customer) (12-14-2021)

I induced the expression of interferon responsive genes by treating HEK293T cells for 24 or 48h with various concentrations of IFNalpha2a. Samples were then analysed by western blot for the detection of an IFN induced protein, which was absent at t=0, clearly induced at 24h and even better expressed at 48h. The lowest amount of IFN analysed here was 3 ng/ml. I also tried IFN beta (cat number HZ-1298) at the same time and the results were also very good, identical.

  • Applications: Cell culture
  • Cell Tissue Type: HEK293T