Alpha Actin Polyklonaler Antikörper

Alpha Actin Polyklonal Antikörper für IF, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

98

Kat-Nr. : 23660-1-AP

Synonyme

ACTA, ACTA1, ACTA1 skeletal Muscle Actin, actin, Actin, alpha skeletal muscle, Alpha Actin, Alpha actin 1, ASMA, CFTD, α actin

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Galerie der Validierungsdaten

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  WB analysis using 23660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 23660-1-AP

  IP Result of anti-alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IF Staining of HepG2 using 23660-1-AP

  Immunofluorescent analysis of HepG2 cells using 23660-1-AP (alpha Actin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of C2C12 using 23660-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23660-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 82 publications below
• IHC: See 1 publications below
• IF: See 17 publications below
• FC: See 1 publications below

Produktinformation

23660-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Alpha Actin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Actin fusion protein Ag19151
• Vollständiger Name: actin, alpha 1, skeletal muscle
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 39-42 kDaa
• GenBank-Zugangsnummer: BC012597
• Gene symbol: ACTA1
• Gene ID (NCBI): 58
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Acta Pharm Sin B; A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis.; Authors - Tailin He
mouse	WB	Transl Res; Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.; Authors - Pengyu Jia
human	WB	Redox Biol; Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response.; Authors - Yan Qi
	WB	Cardiovasc Diabetol; Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X; Authors - Shilong You
human	WB	J Hazard Mater; Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.; Authors - Jing Qin
mouse	WB,IF	Phytomedicine; Aloe-emodin ameliorated MI-induced cardiac remodeling in mice via inhibiting TGF-β/SMAD signaling via up-regulating SMAD7; Authors - Jie Yu

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C

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