Alpha Actin Polyklonaler Antikörper

Alpha Actin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(182)

Rezensionen (1)

Kat-Nr. : 23660-1-AP

Synonyme

ACTA1, α actin, ACTA, ACTA1 skeletal Muscle Actin, Actin alpha skeletal muscle

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  WB analysis using 23660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 23660-1-AP

  IP result of anti-Alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

• 

  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

• 

  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

• 

  IF Staining of mouse heart using 23660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 23660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 23660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of H9C2 using 23660-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using Alpha Actin antibody (23660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of C2C12 using 23660-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23660-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IF/ICC: H9C2-Zellen
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 158 publications below
• IHC: See 4 publications below
• IF: See 20 publications below

Produktinformation

23660-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Alpha Actin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha Actin fusion protein Ag19151
• Vollständiger Name: actin, alpha 1, skeletal muscle
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 39-42 kDaa
• GenBank-Zugangsnummer: BC012597
• Gene symbol: ACTA1
• Gene ID (NCBI): 58
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Metab; QDPR deficiency drives immune suppression in pancreatic cancer; Authors - Ji Liu
mouse,human	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
	WB	J Exp Med; Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenance; Authors - Naijun Miao
mouse	WB	Acta Pharm Sin B; A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis.; Authors - Tailin He
		Redox Biol; Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosis; Authors - Xiangqi Chen
mouse	WB	Transl Res; Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.; Authors - Pengyu Jia

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Arun (Verified Customer) (07-28-2025)

Works great even wat higher dilutions 1:10000

• Applications: Western Blot