ACTA1-Specific Polyklonaler Antikörper

ACTA1-Specific Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 17521-1-AP

Synonyme

ACTA1, Alpha Actin, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
Erfolgreiche Detektion in IHChumanes Skelettmuskelgewebe, humanes Uterusmyomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FC (Intra)C2C12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

17521-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA ACTA1-Specific und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name actin, alpha 1, skeletal muscle
Berechnetes Molekulargewicht 42 kDa
Beobachtetes Molekulargewicht 39-42 kDa
GenBank-ZugangsnummerNM_001100
Gene symbol ACTA1
Gene ID (NCBI) 58
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ACTA1-Specific antibody 17521-1-APProtokoll herunterladen
IHC protocol for ACTA1-Specific antibody 17521-1-APProtokoll herunterladenl
FC protocol for ACTA1-Specific antibody 17521-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

J Clin Invest

Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome.

Authors - Andres Ramirez-Martinez
humanWB

Cancer Lett

Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop.

Authors - Yue Zhang
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng
humanWB

Int J Mol Sci

Neddylation Regulates Class IIa and III Histone Deacetylases to Mediate Myoblast Differentiation

Authors - Hongyi Zhou
ratIF,WB

Aging (Albany NY)

Enriched environment remedies cognitive dysfunctions and synaptic plasticity through NMDAR-Ca2+-Activin A circuit in chronic cerebral hypoperfusion rats.

Authors - Xin Zhang
mouseWB

iScience

A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart

Authors - Aude Angelini

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

ARUN (Verified Customer) (07-29-2025)

works well and specific. It even works for mouse tissues immunofluorescence.

  • Applications: Western Blot