GRK2 Polyklonaler Antikörper

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• KD/KO Validated

GRK2 Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (1)

Kat-Nr. : 13990-1-AP

Synonyme

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, GRK2

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Galerie der Validierungsdaten

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  WB analysis using 13990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13990-1-AP (GRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 13990-1-AP

  IP Result of anti-GRK2 (IP:13990-1-AP, 4ug; Detection:13990-1-AP 1:1000) with HL-60 cells lysate 3040ug.

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  IHC staining of human lymphoma using 13990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 13990-1-AP

  1X10^6 HeLa cells were stained with 0.2ug GRK2 antibody (13990-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13990-1-AP bindet in WB, IP, IHC, FC, CoIP, ELISA GRK2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GRK2 fusion protein Ag5071
• Vollständiger Name: adrenergic, beta, receptor kinase 1
• Berechnetes Molekulargewicht: 689 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC037963
• Gene symbol: ADRBK1
• Gene ID (NCBI): 156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells.; Authors - Qiyin Zhou
human	WB	Nat Commun; Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.; Authors - Fan Yang ; KD Validated
human,mouse	WB,CoIP	J Pharm Anal; Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis; Authors - Jia-Jia Zhang ; KD Validated
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	J Am Heart Assoc; Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension.; Authors - Xuejing Sun ; KD Validated
human	WB	iScience; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia.; Authors - Xin Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Franziska (Verified Customer) (09-11-2023)

Since there are no GRK-2 antibodies specific for C.elegans, I decided to try this one which didn't work very well, there was a lot of unspecific binding however, C.elegans was not mentioned as tested reactivity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: C.elegans

GRK2 Antibody Western Blot validation (1:750 dilution) in C.elegans (Cat no:13990-1-AP)