Beta Tubulin Rekombinanter Antikörper

Beta Tubulin Rekombinant Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch

Anwendung

WB, IP, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2O13

Kat-Nr. : 80713-1-RR

Synonyme

beta 4, beta tubulin, MC1R, TUBB, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3

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Galerie der Validierungsdaten

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  WB analysis using 80713-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 80713-1-RR

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80713-1-RR (Beta Tubulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 80713-1-RR

  IP result of anti-Beta Tubulin(IP:80713-1-RR, 4ug; Detection:66240-1-Ig 1:10000) with HEK-293 cells lysate 1360 ug.

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  IHC staining of rat brain using 80713-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 80713-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80713-1-RR (Beta Tubulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 80713-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 80713-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin antibody (80713-1-RR, Clone: 2O13 ) at dilution of 1:550 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 80713-1-RR

  1X10^6 HEK-293 cells were intracellularly stained with 0.2 ug Anti-Human Beta Tubulin (80713-1-RR, Clone:2O13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, Zebrafischgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: C2C12-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

80713-1-RR bindet in WB, IP, IF, FC, ELISA Beta Tubulin und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Beta Tubulin fusion protein Ag0117
• Vollständiger Name: tubulin, beta 3
• Berechnetes Molekulargewicht: 450 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000748
• Gene symbol: TUBB3
• Gene ID (NCBI): 10381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cells; Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties; Authors - Hidetaka Togo

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C