EIF4E Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EIF4E Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 11149-1-AP

Synonyme

CBP, CBP,EIF4E, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein

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Galerie der Validierungsdaten

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  WB analysis using 11149-1-PBS

  Various lysates were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11149-1-AP

  WB result of EIF4E antibody (11149-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4E transfected HEK-293 cells.

• 

  WB analysis using 11149-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 11149-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11149-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11149-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11149-1-AP (EIF4E antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11149-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 11149-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 11149-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11149-1-AP (EIF4E antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11149-1-AP

  Immunofluorescent analysis of HepG2 cells, using EIF4E antibody 11149-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 11149-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug EIF4E antibody (11149-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11149-1-AP bindet in WB, IHC, IF, FC, ELISA EIF4E und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EIF4E fusion protein Ag1626
• Vollständiger Name: eukaryotic translation initiation factor 4E
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 26-29 kDa
• GenBank-Zugangsnummer: BC012611
• Gene symbol: EIF4E
• Gene ID (NCBI): 1977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins. This is a rabbit polyclonal antibody raised against the full-length human EIF4E.

Publikationen

Species	Application	Title
		Cancer Res; HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.; Authors - Yinghui Li
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB	Mol Ther; RNA m6A methylation regulates the dissemination of cancer cells via modulating expression and membrane localization of β-catenin.; Authors - Jiexin Li
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang
human	WB	Cell Death Dis; Multifunctional viral protein γ34.5 manipulates nucleolar protein NOP53 for optimal viral replication of HSV-1.; Authors - Wen Meng
mouse	WB	ACS Chem Neurosci; Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus; Authors - Yan Sun