CORO1A Polyklonaler Antikörper

CORO1A Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (2)

Kat-Nr. : 17760-1-AP

Synonyme

CLABP, Clipin A, CLIPINA, Clipin-A, CORO1

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  WB analysis of rat brain using 17760-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17760-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17760-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17760-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17760-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17760-1-AP

  IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:3000) with mouse brain tissue lysate 1840 ug.

• 

  IP experiment of mouse spleen using 17760-1-AP

  IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.

• 

  IHC staining of mouse spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 17760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 17760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 17760-1-AP

  Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Jurkat using 17760-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CORO1A (17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HL-60-Zellen, Jurkat-Zellen, Mausmilzgewebe, Maus-Thymusgewebe, Rattenhirngewebe, Rattenmilzgewebe, Ratten-Thymusgewebe
• Erfolgreiche IP: Maushirngewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Rattenmilzgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IF-Fro: Mausmilzgewebe
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

17760-1-AP bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA CORO1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CORO1A fusion protein Ag12139
• Vollständiger Name: coronin, actin binding protein, 1A
• Berechnetes Molekulargewicht: 461 aa, 51 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC110374
• Gene symbol: CORO1A
• Gene ID (NCBI): 11151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

Publikationen

Species	Application	Title
		Br J Pharmacol; PMID: 27128203; Authors - Xianrong Zhang
human	WB	Int Immunopharmacol; Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.; Authors - Fuli Min
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song
rat	WB	PLoS One; Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.; Authors - Feng Guo
human	IF	Theranostics; TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy; Authors - Wen-Jie Gu
mouse	WB	Vet Res; The tryptophan-aspartate (WD) repeat domain of bovine Coronin-1A promotes mycobacterial survival by inhibiting calcium signaling-mediated phagosome-lysosome fusion; Authors - Jing Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jordan (Verified Customer) (12-04-2024)

Antibody worked well for western blot at 1:1000 overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

FH

lucie (Verified Customer) (06-25-2024)

Anti-CORO1A working in immunofluorescence on neutrophils fixed with PFA 2%, at a dilution of 1/200 in BSA 1%.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Neutrophils