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MTCO2 Polyklonaler Antikörper

MTCO2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(155)

Kat-Nr. : 55070-1-AP

Synonyme

COX2, COII, COX 2, COXII, MT CO2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HeLa-Zellen, MCF-7-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)A431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:12000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:4000-1:16000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

55070-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA MTCO2 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Affe, hamster, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name cytochrome c oxidase II
Berechnetes Molekulargewicht 26 kDa
Beobachtetes Molekulargewicht 23-26 kDa
GenBank-ZugangsnummerYP_003024029
Gene symbol MTCO2
Gene ID (NCBI) 4513
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for MTCO2 antibody 55070-1-APProtokoll herunterladen
IHC protocol for MTCO2 antibody 55070-1-APProtokoll herunterladenl
IF protocol for MTCO2 antibody 55070-1-APProtokoll herunterladen
IP protocol for MTCO2 antibody 55070-1-APProtokoll herunterladen
FC protocol for MTCO2 antibody 55070-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Cell

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Authors - Paul K Ziegler
humanWB

Cell Metab

Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.

Authors - Ruchi Masand
humanWB

Cell Metab

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Authors - Yi-Ping Wang
humanWB

Immunity

Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.

Authors - Fangzhou Lou
mouseWB

Cell Res

Active RNA interference in mitochondria.

Authors - Kuanxing Gao
humanWB

Mol Cell

Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma

Authors - Linyu Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Matthieu (Verified Customer) (09-24-2025)

Band is clearly visible at the correct size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: human fibroblasts
FH

Manon (Verified Customer) (09-24-2025)

vey nice band

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Fibroblasts
FH

Emilie (Verified Customer) (09-24-2025)

Bands appeared sharp and well defined under the chosen dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fibroblasts
FH

Vignesh (Verified Customer) (09-03-2025)

It's a good product with reduced or no nonspecific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293
FH

Lenie (Verified Customer) (07-18-2025)

The IF staining and WB with the followed protocol of ProteinTech did not work on zebrafish tissue, even it describes on the site it should. Is there a possibility to send a working protocol about this Abies for zebrafish? Thanks. The bond which shows is a human patient sample.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/2000 (WB) and 1/200 (IF)
  • Cell Tissue Type: Zebrafish
MTCO2 Antibody Western Blot,Immunofluorescence validation (1/2000 (WB) and 1/200 (IF) dilution) in Zebrafish (Cat no:55070-1-AP)
FH

Baptiste (Verified Customer) (05-23-2025)

Working perfectly

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Lymphoblastoid
MTCO2 Antibody Western Blot validation (1:10000 dilution) in Lymphoblastoid (Cat no:55070-1-AP)
FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562 and KCL22
FH

Maria (Verified Customer) (03-11-2021)

MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
MTCO2 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:55070-1-AP)