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MTCO2 Polyklonaler Antikörper
MTCO2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (4)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 55070-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A431-Zellen, HeLa-Zellen, MCF-7-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | A431-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:4000-1:16000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 140 publications below |
IHC | See 10 publications below |
IF | See 6 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Produktinformation
55070-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA MTCO2 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Affe, hamster, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | cytochrome c oxidase II |
Berechnetes Molekulargewicht | 26 kDa |
Beobachtetes Molekulargewicht | 23-26 kDa |
GenBank-Zugangsnummer | YP_003024029 |
Gene symbol | MTCO2 |
Gene ID (NCBI) | 4513 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for MTCO2 antibody 55070-1-AP | Protokoll herunterladen |
IHC protocol for MTCO2 antibody 55070-1-AP | Protokoll herunterladenl |
IF protocol for MTCO2 antibody 55070-1-AP | Protokoll herunterladen |
IP protocol for MTCO2 antibody 55070-1-AP | Protokoll herunterladen |
FC protocol for MTCO2 antibody 55070-1-AP | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. | ||
Cell Metab Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits. | ||
Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. | ||
Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. | ||
Mol Cell Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Matthieu (Verified Customer) (09-24-2025) | Band is clearly visible at the correct size
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FH Manon (Verified Customer) (09-24-2025) | vey nice band
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FH Emilie (Verified Customer) (09-24-2025) | Bands appeared sharp and well defined under the chosen dilution.
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FH Vignesh (Verified Customer) (09-03-2025) | It's a good product with reduced or no nonspecific bands.
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FH Lenie (Verified Customer) (07-18-2025) | The IF staining and WB with the followed protocol of ProteinTech did not work on zebrafish tissue, even it describes on the site it should. Is there a possibility to send a working protocol about this Abies for zebrafish? Thanks. The bond which shows is a human patient sample.
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FH Baptiste (Verified Customer) (05-23-2025) | Working perfectly
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FH Edward (Verified Customer) (11-15-2022) | Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.
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FH Maria (Verified Customer) (03-11-2021) | MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.
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