• Featured Product
  • KD/KO Validated

CPT1A Polyklonaler Antikörper

CPT1A Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(426)

Kat-Nr. : 15184-1-AP

Synonyme

Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1, CPT-1A



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen, Mauslebergewebe, Mausmilzgewebe, Rattenlebergewebe
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, HepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:300-1:1200
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15184-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA CPT1A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Ente, Hausschwein, Huhn, Maus, Ratte, Rind, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CPT1A fusion protein Ag7202
Vollständiger Name carnitine palmitoyltransferase 1A (liver)
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht 86 kDa
GenBank-ZugangsnummerBC000185
Gene symbol CPT1A
Gene ID (NCBI) 1374
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPT1A, also named CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. CPT1A gene is localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active form of related liver-type carnitine palmitoyltransferase I (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CPT1A antibody 15184-1-APProtokoll herunterladen
IHC protocol for CPT1A antibody 15184-1-APProtokoll herunterladenl
IF protocol for CPT1A antibody 15184-1-APProtokoll herunterladen
IP protocol for CPT1A antibody 15184-1-APProtokoll herunterladen
FC protocol for CPT1A antibody 15184-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
mouseWB

Nature

High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.

Authors - Semir Beyaz
mouseWB,IF

Cell Metab

Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis.

Authors - Joanna Kalucka
mouseWB

Cell

Mitochondrial Priming by CD28.

Authors - Ramon I Klein Geltink
  • KD Validated
mouseWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang

Cell Metab

Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity.

Authors - Mauro Corrado

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

YINGJIAN (Verified Customer) (08-07-2025)

It yields a clear signal with minimal background in both Western blot and immunohistochemistry assays

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: liver
CPT1A Antibody Western Blot,Immunohistochemistry validation (1:5000 dilution) in liver (Cat no:15184-1-AP)
FH

Yuan (Verified Customer) (09-12-2024)

We suffered from poor images with CPT1 antibodies purchased from other companies. This antibody saved us. The top band is CPT1A at 80 kDa and the bottom one is GAPDH in the attached graph.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500 in 1% BSA/TBST
  • Cell Tissue Type: Human primary dermal fibroblast
CPT1A Antibody Western Blot validation (1:500 in 1% BSA/TBST dilution) in Human primary dermal fibroblast (Cat no:15184-1-AP)
FH

Tom (Verified Customer) (05-17-2021)

Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293
CPT1A Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:15184-1-AP)
FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
  • Cell Tissue Type: mice lung macrophages cell line
CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)