CPT1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CPT1A Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

293

Rezensionen (2)

Kat-Nr. : 15184-1-AP

Synonyme

CPT I, CPT1, CPT1 L, CPT1A, CPTI L, L CPT1

---

Galerie der Validierungsdaten

• 

  WB analysis of A549 cells using 15184-1-AP

  WB results of CPT1A antibody (1:1000) with shCPT1A-A549 and shCPT1A-HepG2 cells. shControl-A549 and shControl-HepG2 as positive control.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15184-1-AP (CPT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15184-1-AP

  IP result of anti-CPT1A (IP:15184-1-AP, 4ug; Detection:15184-1-AP 1:1000) with HepG2 cells lysate 3000 ug.

• 

  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15184-1-AP (CPT1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15184-1-AP (CPT1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15184-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15184-1-AP (CPT1A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15184-1-AP

  Immunofluorescent analysis of -20°C Ethanol fixed HepG2 cells stained for CPT1A rabbit pAb (15184-1-AP) and KRT18 mouse mAb (66187-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 15184-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) for 66187-1-Ig.

• 

  FC experiment of HeLa using 15184-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CPT1A (15184-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HepG2-Zellen, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe, U-251-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 17 publications below
• WB: See 267 publications below
• IHC: See 27 publications below
• IF: See 16 publications below
• IP: See 2 publications below
• FC: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

15184-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA CPT1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ente, Hausschwein, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPT1A fusion protein Ag7202
• Vollständiger Name: carnitine palmitoyltransferase 1A (liver)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC000185
• Gene symbol: CPT1A
• Gene ID (NCBI): 1374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. It is Localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Nature; High-fat diet enhances stemness and tumorigenicity of intestinal progenitors.; Authors - Semir Beyaz
mouse	WB,IF	Cell Metab; Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis.; Authors - Joanna Kalucka
mouse	WB,FC	Cell; Mitochondrial Priming by CD28.; Authors - Ramon I Klein Geltink ; KD Validated
mouse	IHC	Cell Metab; SGLT2 Inhibition Mediates Protection from Diabetic Kidney Disease by Promoting Ketone Body-Induced mTORC1 Inhibition.; Authors - Issei Tomita
		Cell Metab; Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity.; Authors - Mauro Corrado

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tom (Verified Customer) (05-17-2021)

Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

CPT1A Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:15184-1-AP)

FH

Linlin (Verified Customer) (05-08-2019)

This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 2ug per pulldown; 1000x for immunoblot analysis
• Cell Tissue Type: mice lung macrophages cell line

CPT1A Antibody Western Blot,Immunoprecipitation, validation (2ug per pulldown; 1000x for immunoblot analysis dilution) in mice lung macrophages cell line (Cat no:15184-1-AP)

Verwandte Produkte

CPT1A Monoclonal antibody

66039-1-Ig

CPT1C-specific Polyclonal antibody

12969-1-AP

CPT1B-specific Polyclonal antibody

22170-1-AP

FASN Polyclonal antibody

10624-2-AP

SREBF1 Polyclonal antibody

14088-1-AP