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CPT1A Polyklonaler Antikörper
CPT1A Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (6)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15184-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HepG2-Zellen, A549-Zellen, HeLa-Zellen, MCF-7-Zellen, Mauslebergewebe, Mausmilzgewebe, Rattenlebergewebe |
Erfolgreiche IP | HepG2-Zellen |
Erfolgreiche Detektion in IHC | humanes Leberkarzinomgewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen, HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:300-1:1200 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 20 publications below |
WB | See 384 publications below |
IHC | See 40 publications below |
IF | See 31 publications below |
IP | See 3 publications below |
CoIP | See 2 publications below |
ChIP | See 1 publications below |
Produktinformation
15184-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA CPT1A und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Ente, Hausschwein, Huhn, Maus, Ratte, Rind, Zebrafisch, Ziege |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | CPT1A fusion protein Ag7202 |
Vollständiger Name | carnitine palmitoyltransferase 1A (liver) |
Berechnetes Molekulargewicht | 88 kDa |
Beobachtetes Molekulargewicht | 86 kDa |
GenBank-Zugangsnummer | BC000185 |
Gene symbol | CPT1A |
Gene ID (NCBI) | 1374 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CPT1A, also named CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. CPT1A gene is localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active form of related liver-type carnitine palmitoyltransferase I (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for CPT1A antibody 15184-1-AP | Protokoll herunterladen |
IHC protocol for CPT1A antibody 15184-1-AP | Protokoll herunterladenl |
IF protocol for CPT1A antibody 15184-1-AP | Protokoll herunterladen |
IP protocol for CPT1A antibody 15184-1-AP | Protokoll herunterladen |
FC protocol for CPT1A antibody 15184-1-AP | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Cell Metab Quiescent Endothelial Cells Upregulate Fatty Acid β-Oxidation for Vasculoprotection via Redox Homeostasis. | ||
Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH YINGJIAN (Verified Customer) (08-07-2025) | It yields a clear signal with minimal background in both Western blot and immunohistochemistry assays
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FH Yuan (Verified Customer) (09-12-2024) | We suffered from poor images with CPT1 antibodies purchased from other companies. This antibody saved us. The top band is CPT1A at 80 kDa and the bottom one is GAPDH in the attached graph.
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FH Tom (Verified Customer) (05-17-2021) | Antibody used at 1/1000 for 24 hours at 4 degrees. Non-specific bands were also present upon imaging.
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FH Linlin (Verified Customer) (05-08-2019) | This CPT1A antibody is ideal for precipitation of CPT1A in cell lysates and immunoblot analysis of CPT1A. Recommend.
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