CUL4A-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CUL4A-Specific Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 14851-1-AP

Synonyme

CUL 4A, CUL4A, CUL4A-Specific, cullin 4A

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Galerie der Validierungsdaten

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  WB analysis using 14851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 14851-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CUL4A-Specific (14851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

14851-1-AP bindet in WB, IP, IHC, FC, ELISA CUL4A-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cullin 4A
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 77-88 kDa
• GenBank-Zugangsnummer: NM_003589
• Gene symbol: CUL4A
• Gene ID (NCBI): 8451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A,but not CUL4B.

Publikationen

Species	Application	Title
human	WB,IHC	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng ; KD Validated
mouse	WB	Nat Immunol; COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.; Authors - Menon Suchithra S
mouse	WB	Mol Cell; Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.; Authors - Peng Jiang ; KD Validated
human	WB	Dev Cell; AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.; Authors - Manuela Antonioli ; KD Validated
mouse	WB	Nat Commun; CSN6 drives carcinogenesis by positively regulating Myc stability.; Authors - Jian Chen
mouse	WB	Redox Biol; Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.; Authors - Ji Zhang

Verwandte Produkte

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11612-1-AP

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12916-1-AP

DDB2 Polyclonal antibody

10431-1-AP

RBX1 Polyclonal antibody

14895-1-AP

PCNA Polyclonal antibody

10205-2-AP

DTL Polyclonal antibody

12896-1-AP

CUL2 Polyclonal antibody

10981-2-AP

RBX1 Polyclonal antibody

14895-1-AP

NEDD8 Polyclonal antibody

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VPRBP Polyclonal antibody

11612-1-AP