calreticulin Polyklonaler Antikörper

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• KD/KO Validated

calreticulin Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 27298-1-AP

Synonyme

CALR, Calregulin, calreticulin, cC1qR, CRP55, CRT, CRTC, ERp60, grp60, HACBP, RO, SSA

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 27298-1-AP

  WB result of Calreticulin antibody (27298-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calreticulin transfected HeLa cells.

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  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27298-1-AP (Calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 27298-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27298-1-AP (calreticulin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 27298-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 27298-1-AP (calreticulin Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).

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  IF Staining of HepG2 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using calreticulin antibody (27298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of SKOV-3 using 27298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Calreticulin antibody (27298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 27298-1-AP

  1X10^6 Jurkat cells were stained with 0.2 ug Anti-Human Calreticulin (27298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Maushirngewebe, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 23 publications below
• FC: See 2 publications below

Produktinformation

27298-1-AP bindet in WB, IHC, IF, FC, ELISA calreticulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: calreticulin fusion protein Ag26064
• Vollständiger Name: calreticulin
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC002500
• Gene symbol: CALR
• Gene ID (NCBI): 811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Publikationen

Species	Application	Title
human	WB	Cell Metab; Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK.; Authors - Mengqi Li
mouse	IF	Nat Commun; In situ generation of micrometer-sized tumor cell-derived vesicles as autologous cancer vaccines for boosting systemic immune responses; Authors - Yuxin Guo
mouse	FC,IF	Nat Commun; Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.; Authors - Yanjuan Huang
mouse	IF	ACS Appl Mater Interfaces; Cascade-Responsive Hierarchical Nanosystems for Multisite Specific Drug Exposure and Boosted Chemoimmunotherapy; Authors - Junmei Zhang
human,mouse	IF,WB	ACS Appl Mater Interfaces; A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment; Authors - Ao Chen
mouse	WB	ACS Appl Mater Interfaces; Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform; Authors - Xianglong Yu