DIS3L2 Monoklonaler Antikörper

DIS3L2 Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2C9F2

Kat-Nr. : 67623-1-Ig

Synonyme

DIS3 like exonuclease 2, DIS3-like exonuclease 2, EC:3.1.13.-, FAM6A, hDIS3L2

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  WB analysis using 67623-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 67623-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  FC experiment of Jurkat using 67623-1-Ig

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DIS3L2 Monoclonal antibody (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U2OS-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

67623-1-Ig bindet in WB, FC (Intra), ELISA DIS3L2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DIS3L2 fusion protein Ag29992
• Vollständiger Name: DIS3 mitotic control homolog (S. cerevisiae)-like 2
• Berechnetes Molekulargewicht: 885 aa, 99 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 99 kda
• GenBank-Zugangsnummer: BC030113
• Gene symbol: DIS3L2
• Gene ID (NCBI): 129563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; LINE-1 mRNA 3' end dynamics shape its biology and retrotransposition potential; Authors - Damian M Janecki
human	WB	Cell Rep; Cytoplasmic DIS3 is an exosome-independent endoribonuclease with catalytic activity toward circular RNAs; Authors - Claudia Latini