GABARAPL1-Specific Polyklonaler Antikörper

GABARAPL1-Specific Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 18721-1-AP

Synonyme

GABARAPL1, APG8 LIKE, APG8L, ATG8, ATG8L

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  WB analysis using 18721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18721-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 18721-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 18721-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL1-Specific Polyclonal antibody (18721-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Herzgewebe, humanes Milzgewebe, Mausnierengewebe, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

18721-1-AP bindet in WB, IHC, FC (Intra), ELISA GABARAPL1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GABA(A) receptor-associated protein like 1
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM_031412
• Gene symbol: GABARAPL1
• Gene ID (NCBI): 23710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle. And at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1 and doesn't recognize the recombinant GABARAP and GABARAPL2.

Publikationen

Species	Application	Title
human	WB	Autophagy; GABARAPL1 antibodies: target one protein, get one free!; Authors - Le Grand Jaclyn Nicole JN
human	WB	Sci Rep; Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.; Authors - Alexandra Boeske
human	WB	BMC Musculoskelet Disord; MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis; Authors - Pengfei Xu