JMJD6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

JMJD6 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 16476-1-AP

Synonyme

Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, EC:1.14.11.-, JmjC domain-containing protein 6, Jumonji domain-containing protein 6, KIAA0585

---

• 

  WB analysis of mouse liver using 16476-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 16476-1-AP

  IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug.

• 

  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16476-1-AP (JMJD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16476-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of A549 using 16476-1-AP

  1x10^6 A549 cells were intracellularly stained with 0.4 ug JMJD6 Polyclonal antibody (16476-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

16476-1-AP bindet in WB, IHC, IF, FC (Intra), IP, CoIP, ChIP, ELISA JMJD6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JMJD6 fusion protein Ag9590
• Vollständiger Name: jumonji domain containing 6
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC066654
• Gene symbol: JMJD6
• Gene ID (NCBI): 23210
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	WB,IP,ChIP	Mol Cell; JMJD6 Licenses ERα-Dependent Enhancer and Coding Gene Activation by Modulating the Recruitment of the CARM1/MED12 Co-activator Complex.; Authors - Wei-Wei Gao ; KD Validated
mouse,human	IHC	Front Immunol; Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression; Authors - Zhao Guang
human	WB	PLoS Pathog; JMJD6 negatively regulates cytosolic RNA induced antiviral signaling by recruiting RNF5 to promote activated IRF3 K48 ubiquitination.; Authors - Wei Zhang ; KO Validated
chicken	WB	Vet Res; The long non-coding RNA lncRNA-DRNR enhances infectious bronchitis virus replication by targeting chicken JMJD6 and modulating interferon-stimulated genes expression via the JAK-STAT signalling pathway; Authors - Wenjun Yan
human	WB	Chem Sci; A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation; Authors - Yi-Cheng Sin
human	WB	Theranostics; PRMT6-mediated ADMA promotes p62 phase separation to form a negative feedback loop in ferroptosis; Authors - Lifeng Feng