KIF15 Polyklonaler Antikörper

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• KD/KO Validated

KIF15 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 55407-1-AP

Synonyme

hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15, KLP2

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  WB analysis of HEK-293 using 55407-1-AP

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells.

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  WB analysis of Jurkat using 55407-1-AP

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells.

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  WB analysis using 55407-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 55407-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 55407-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 55407-1-AP

  IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human testis using 55407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 55407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HEK-293 using 55407-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, MDA-MB-453s-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 28 publications below
• IHC: See 14 publications below
• IF: See 8 publications below

Produktinformation

55407-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA KIF15 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: kinesin family member 15
• Berechnetes Molekulargewicht: 160 kDa
• Beobachtetes Molekulargewicht: 150-165 kDa
• GenBank-Zugangsnummer: NM_020242
• Gene symbol: KIF15
• Gene ID (NCBI): 56992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

Publikationen

Species	Application	Title
human	WB	Am J Respir Crit Care Med; Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis; Authors - David Zhang
human	WB,IHC	Cancer Res; KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.; Authors - Lin Gao ; KD Validated
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
human	WB,IHC	Cell Physiol Biochem; Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.; Authors - Yuan Qiao ; KD Validated
mouse	WB	Front Cell Dev Biol; Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.; Authors - Sai Aung Phyo
human	WB,IHC,IF	Transl Oncol; METTL3 methylated KIF15 promotes nasopharyngeal carcinoma progression and radiation resistance by blocking ATG7-mediated autophagy through the activation of STAT3 pathway; Authors - Siwei Li