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Cytokeratin 19 Monoklonaler Antikörper
Cytokeratin 19 Monoklonal Antikörper für WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
3G1E4
Kat-Nr. : 60187-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | MCF-7-Zellen, HaCaT-Zellen, HeLa-Zellen, Maus-Kolongewebe, PC-3-Zellen, Hausschwein-Kolongewebe, Ratten-Kolongewebe, T-47D-Zellen | 
| Erfolgreiche Detektion in IHC-Autostainer | humanes Kolongewebe | 
| Erfolgreiche Detektion in IHC | humanes Kolongewebe,  humanes Mammakarzinomgewebe,  humanes Lungenkarzinomgewebe,  humanes Ösophaguskarzinomgewebe,  humanes Magenkrebsgewebe,  humanes Schilddrüsenkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF-P | humanes Mammakarzinomgewebe | 
| Erfolgreiche Detektion in IF-Fro | mouse breast cancer | 
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 | 
| Immunhistochemie (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:12500-1:50000 | 
| Immunhistochemie (IHC) | IHC : 1:1000-1:51200 | 
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 | 
| Immunfluoreszenz (IF)-FRO | IF-FRO : 1:200-1:800 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 3 publications below | 
| IHC | See 3 publications below | 
| IF | See 10 publications below | 
Produktinformation
60187-1-Ig bindet in WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA Cytokeratin 19 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Maus | 
| Wirt / Isotyp | Maus / IgG2b | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | Cytokeratin 19 fusion protein Ag7407 | 
| Vollständiger Name | keratin 19 | 
| Berechnetes Molekulargewicht | 44 kDa | 
| Beobachtetes Molekulargewicht | 48 kDa | 
| GenBank-Zugangsnummer | BC002539 | 
| Gene symbol | Cytokeratin 19 | 
| Gene ID (NCBI) | 3880 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Cytokeratin 19 antibody 60187-1-Ig | Protokoll herunterladen | 
| IHC protocol for Cytokeratin 19 antibody 60187-1-Ig | Protokoll herunterladenl | 
| IF protocol for Cytokeratin 19 antibody 60187-1-Ig | Protokoll herunterladen | 
| FC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Nat Commun Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy | ||
| Stem Cells Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells. | ||
| Stem Cell Res Ther DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis | ||
| Breast Cancer Res Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors | ||
| Commun Biol Chemerin attenuates acute kidney injury by inhibiting ferroptosis via the AMPK/NRF2/SLC7A11 axis | ||
| Biomed Pharmacother Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Kis (Verified Customer) (02-21-2025) | This antibody is working. well for IF anlysis in human and mouse liver samples. 
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