Cytokeratin 7 Polyklonaler Antikörper

Cytokeratin 7 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 15539-1-AP

Synonyme

KRT7, CK 7, CK7, CK-7, Cytokeratin,Keratin



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A431-Zellen, HepG2-Zellen, Maus-Blasengewebe, Mauslebergewebe, Mauslungengewebe, Ratten-Blasengewebe, Rattenlebergewebe, Rattenlungengewebe
Erfolgreiche Detektion in IHChumanes Pankreasgewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Lungenkarzinomgewebe, humanes Lungengewebe, humanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe, Mausnierengewebe, Maus-Pankreasgewebe, Rattennierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMaus-Pankreasgewebe, Rattenlebergewebe
Erfolgreiche Detektion in IF-Fromouse breast cancer
Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:1000-1:30000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)-FROIF-FRO : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15539-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Cytokeratin 7 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Cytokeratin 7 fusion protein Ag7895
Vollständiger Name keratin 7
Berechnetes Molekulargewicht 469 aa, 51 kDa
Beobachtetes Molekulargewicht 51 kDa
GenBank-ZugangsnummerBC002700
Gene symbol Cytokeratin 7
Gene ID (NCBI) 3855
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytokeratin 7 (CK7) is a type II keratin protein that is a principal constituent of the intermediate filament cytoskeleton. It is primarily expressed in simple epithelia lining the cavities of internal organs, glandular ducts, and blood vessels. Abnormal expression of CK7 has been linked to various pathological conditions, including cancer. Overexpression of CK7 promotes tumor progression and metastasis in different human cancers, and its suppression leads to rapid tumor regression, highlighting its potential as a therapeutic target CK7 is also involved in inhibiting interferon-dependent interphase, promoting DNA synthesis, initiating translation possibly through interaction with p150 (the largest subunit of eukaryotic translation initiation factor 3), and interacting with G protein-coupled estrogen receptor 1 (GPER1), which activates several signaling pathways. In the context of cancer diagnosis, CK7 is used as a marker to distinguish between different types of carcinomas. It is expressed in most adenocarcinomas, particularly those of the lung and colorectal origin, and is useful in differentiating primary ovarian carcinoma from metastatic colorectal carcinoma. CK7 expression has also been studied as a predictor of an unfavorable prognosis in colorectal carcinoma.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Cytokeratin 7 antibody 15539-1-APProtokoll herunterladen
IHC protocol for Cytokeratin 7 antibody 15539-1-APProtokoll herunterladenl
IF protocol for Cytokeratin 7 antibody 15539-1-APProtokoll herunterladen
FC protocol for Cytokeratin 7 antibody 15539-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Cell

Neurulation of the cynomolgus monkey embryo achieved from 3D blastocyst culture

Authors - Jinglei Zhai
mouseIHC

Cancer Cell

Promotion of cholangiocarcinoma growth by diverse cancer-associated fibroblast subpopulations.

Authors - Silvia Affo
humanIF

J Nanobiotechnology

GelMA loaded with exosomes from human minor salivary gland organoids enhances wound healing by inducing macrophage polarization

Authors - Jiaying Qian
humanIF

Cell Death Dis

α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation

Authors - Hao Wang
humanWB,IHC,IF

Apoptosis

PANoptosis subtypes predict prognosis and immune efficacy in gastric cancer

Authors - Zitao Liu
humanWB,IHC,IF

Cell Death Dis

LIF upregulates poFUT1 expression and promotes trophoblast cell migration and invasion at the fetal-maternal interface.

Authors - S Liu