LCK Monoklonaler Antikörper

LCK Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2D10C2

Kat-Nr. : 60162-1-Ig

Synonyme

2D10C2, EC:2.7.10.2, Lck p56, Leukocyte C-terminal Src kinase, LSK

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  WB analysis of pig thymus using 60162-1-Ig

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60162-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat thymus using 60162-1-Ig

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60162-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 60162-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60162-1-Ig (LCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60162-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60162-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60162-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60162-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60162-1-Ig (LCK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60162-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60162-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 60162-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 60162-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCK antibody (60162-1-Ig, Clone: 2D10C2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 60162-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human LCK (60162-1-Ig, Clone:2D10C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NK-92 cells, Jurkat-Zellen, MOLT-4-Zellen, Hausschwein-Thymusgewebe, Ramos-Zellen, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

60162-1-Ig bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA LCK und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LCK fusion protein Ag7694
• Vollständiger Name: lymphocyte-specific protein tyrosine kinase
• Berechnetes Molekulargewicht: 539 aa, 56 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC013200
• Gene symbol: LCK
• Gene ID (NCBI): 3932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCK(Lymphocyte cell-specific protein-tyrosine kinase) is also named as LSK,p56-LCK and a nonreceptor protein-tyrosine kinase of the SRConcogene family that is involved in transduction of T-cell receptor-mediated activation.It is implicated in the control of lymphocyte growth by virtue of its overexpression in some lymphoid malignancies and its transforming activity in heterologous systems(PMID:2784463).It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB,IHC	Int J Gen Med; Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis.; Authors - Chao Wang
human	WB,IHC	Cancer Lett; Distinct Immunophenotypic Profiles and Neutrophil Heterogeneity in Colorectal Cancer; Authors - Minghua Bai
human	WB,CoIP	Mol Med; c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation; Authors - Nan Yang