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LC3 Polyklonaler Antikörper

LC3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1663)

Kat-Nr. : 14600-1-AP

Synonyme

MAP1LC3B, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, MAP1 light chain 3-like protein 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, mit Chloroquin behandelte HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Gliomgewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCmit Chloroquin behandelte HeLa-Zellen, mit Chloroquin behandelte HepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:8000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:250-1:1000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14600-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA LC3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, hamster, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LC3 fusion protein Ag6144
Vollständiger Name microtubule-associated protein 1 light chain 3 beta
Berechnetes Molekulargewicht 15 kDa
Beobachtetes Molekulargewicht 14-18 kDa
GenBank-ZugangsnummerBC067797
Gene symbol LC3B
Gene ID (NCBI) 81631
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for LC3 antibody 14600-1-APProtokoll herunterladen
IHC protocol for LC3 antibody 14600-1-APProtokoll herunterladenl
IF protocol for LC3 antibody 14600-1-APProtokoll herunterladen
FC protocol for LC3 antibody 14600-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Signal Transduct Target Ther

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy

Authors - Yang Meng
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
mouseWB

Gastroenterology

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Authors - Decai Wang
mouseWB,IF

Nat Cell Biol

Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells

Authors - Huafeng Zhang
mouseWB,IF

Bioact Mater

Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway

Authors - Tian Qin
humanWB

Acta Neuropathol

C9orf72 intermediate repeats are associated with corticobasal degeneration, increased C9orf72 expression and disruption of autophagy.

Authors - Christopher P Cali

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

A (Verified Customer) (07-15-2025)

Used for caco2 cells and animal colon tissue

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1 to 1000
FH

Aditya (Verified Customer) (11-01-2024)

great with ecl

  • Applications: Western Blot
FH

David (Verified Customer) (01-02-2024)

Good antibody, single band at the predicted molecular weight, no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y lysate
FH

Hala (Verified Customer) (05-09-2023)

works very good in milk5%

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HepG2
LC3 Antibody Western Blot validation (1/2000 dilution) in HepG2 (Cat no:14600-1-AP)
FH

Hala (Verified Customer) (04-28-2023)

works very good with milk blocking

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: HepG2
FH

Zhihao (Verified Customer) (07-25-2022)

We could detect one form of LC3 in our cell samples, which should be two.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: GFP-Parkin transfected HeLa cell
LC3 Antibody Western Blot validation (1:1000 dilution) in GFP-Parkin transfected HeLa cell (Cat no:14600-1-AP)
FH

S (Verified Customer) (12-31-2021)

I have tried three different LC3B antibodies from three different companies so far. This antibody is by far the best (cost efficient as well)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HCT116
LC3 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:14600-1-AP)
FH

YING (Verified Customer) (09-25-2021)

The antibody works very well. Distinguished LC3-I and LC3-II bands. An increase of LC3-II level in response to treatment with bafilomycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
  • Cell Tissue Type: mouse cells
LC3 Antibody Western Blot validation (1:1500 dilution) in mouse cells (Cat no:14600-1-AP)
FH

Eric (Verified Customer) (03-13-2021)

Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Microglia
LC3 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia (Cat no:14600-1-AP)
FH

Elzbieta (Verified Customer) (09-23-2020)

The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse liver, human HepG2 cells
FH

An (Verified Customer) (09-17-2020)

Used for IHC on zebrafish cryosections of eyes. Worked well.

  • Applications: Immunohistochemistry
FH

David (Verified Customer) (03-30-2020)

Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: SH-SY5Y