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LC3 Polyklonaler Antikörper
LC3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (6)
Anwendung
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14600-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, mit Chloroquin behandelte HeLa-Zellen, Maushirngewebe, Rattenhirngewebe |
Erfolgreiche Detektion in IHC | humanes Lebergewebe, humanes Gliomgewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | mit Chloroquin behandelte HeLa-Zellen, mit Chloroquin behandelte HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:8000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:250-1:1000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 1474 publications below |
IHC | See 168 publications below |
IF | See 456 publications below |
IP | See 6 publications below |
ELISA | See 1 publications below |
CoIP | See 5 publications below |
Produktinformation
14600-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA LC3 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Affe, hamster, Huhn, Kaninchen, Maus, Ratte, Zebrafisch, Ziege |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | LC3 fusion protein Ag6144 |
Vollständiger Name | microtubule-associated protein 1 light chain 3 beta |
Berechnetes Molekulargewicht | 15 kDa |
Beobachtetes Molekulargewicht | 14-18 kDa |
GenBank-Zugangsnummer | BC067797 |
Gene symbol | LC3B |
Gene ID (NCBI) | 81631 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for LC3 antibody 14600-1-AP | Protokoll herunterladen |
IHC protocol for LC3 antibody 14600-1-AP | Protokoll herunterladenl |
IF protocol for LC3 antibody 14600-1-AP | Protokoll herunterladen |
FC protocol for LC3 antibody 14600-1-AP | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy | ||
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells | ||
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
Bioact Mater Local delivery of EGFR+NSCs-derived exosomes promotes neural regeneration post spinal cord injury via miR-34a-5p/HDAC6 pathway | ||
Acta Neuropathol C9orf72 intermediate repeats are associated with corticobasal degeneration, increased C9orf72 expression and disruption of autophagy. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH A (Verified Customer) (07-15-2025) | Used for caco2 cells and animal colon tissue
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FH Aditya (Verified Customer) (11-01-2024) | great with ecl
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FH David (Verified Customer) (01-02-2024) | Good antibody, single band at the predicted molecular weight, no background.
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FH Hala (Verified Customer) (05-09-2023) | works very good in milk5%
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FH Hala (Verified Customer) (04-28-2023) | works very good with milk blocking
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FH Zhihao (Verified Customer) (07-25-2022) | We could detect one form of LC3 in our cell samples, which should be two.
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FH S (Verified Customer) (12-31-2021) | I have tried three different LC3B antibodies from three different companies so far. This antibody is by far the best (cost efficient as well)
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FH YING (Verified Customer) (09-25-2021) | The antibody works very well. Distinguished LC3-I and LC3-II bands. An increase of LC3-II level in response to treatment with bafilomycin A.
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FH Eric (Verified Customer) (03-13-2021) | Worked very well. I was able to get the LC3A/B bands on western blot using a 12.5% gel and prominent bands on a 8% gel. IF imaging worked fairly well, I used 1:100 which was likely too low so I will repeat at higher but got signal nonetheless. This performed better than some of the other LC3 antibodies we tested.
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FH Elzbieta (Verified Customer) (09-23-2020) | The antibody works very nicely in WB. Clear signal, no aspecific bands. Our lab discarded other products and switched to this antibody.
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FH An (Verified Customer) (09-17-2020) | Used for IHC on zebrafish cryosections of eyes. Worked well.
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FH David (Verified Customer) (03-30-2020) | Very nice visualisation of LC3-I and LC3-II. Run on 20% gel and significant changes in response to treatment with bafilomycin A and concanamycin A.
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