MDH1 Monoklonaler Antikörper

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• KD/KO Validated

MDH1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1F9A2

Kat-Nr. : 66505-1-Ig

Synonyme

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

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  WB analysis using 66505-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66505-1-Ig

  WB result of MDH1 antibody (66505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

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  WB analysis of RAW 264.7 using 66505-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66505-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66505-1-Ig (MDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 66505-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66505-1-Ig (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 66505-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66505-1-Ig (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66505-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66505-1-Ig (MDH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HEK-293 using 66505-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (66505-1-Ig, Clone:1F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

66505-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA MDH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: MDH1 fusion protein Ag8744
• Vollständiger Name: malate dehydrogenase 1, NAD (soluble)
• Berechnetes Molekulargewicht: 334 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-37 kDa
• GenBank-Zugangsnummer: BC001484
• Gene symbol: MDH1
• Gene ID (NCBI): 4190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme. (PMID:10693743)

Publikationen

Species	Application	Title
human	IP	Cell Mol Biol Lett; IDH1/MDH1 deacetylation promotes acute liver failure by regulating NETosis; Authors - Yukun Wang
mouse	WB	J Proteome Res; Characteristics of Myocardial Structure and Central Carbon Metabolism during the Early and Compensatory Stages of Cardiac Hypertrophy; Authors - Yuan Chen