MYH1-motor domain Polyklonaler Antikörper

MYH1-motor domain Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 22282-1-AP

Synonyme

MYH1, Myosin-1, Myosin 1, MyHC-IIx/d, MyHC-2x

---

• 

  WB analysis of human skeletal muscle using 22282-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 22282-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22282-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of C2C12 using 22282-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22282-1-AP bindet in WB, IHC, FC (Intra), ELISA MYH1-motor domain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: myosin, heavy chain 1, skeletal muscle, adult
• Berechnetes Molekulargewicht: 223 kDa
• Beobachtetes Molekulargewicht: 223 kDa
• GenBank-Zugangsnummer: NM_005963
• Gene symbol: MYH1
• Gene ID (NCBI): 4619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Moonhee (Verified Customer) (11-18-2021)

It works very well. it can be diluted more for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RCC cells