SMMHC Polyklonaler Antikörper

SMMHC Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Rezensionen (1)

Kat-Nr. : 21404-1-AP

Synonyme

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse uterus using 21404-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21404-1-AP (SMMHC antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21404-1-AP (SMMHC antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse heart using 21404-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 21404-1-AP (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 21404-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human SMMHC (21404-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Uterusgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Skelettmuskelgewebe, Maus-Augengewebe, Ratten-Augengewebe, Ratten-Blasengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe
• Erfolgreiche Detektion in FC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Produktinformation

21404-1-AP bindet in WB, IHC, IF, FC, ELISA SMMHC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMMHC fusion protein Ag16114
• Vollständiger Name: myosin, heavy chain 11, smooth muscle
• Berechnetes Molekulargewicht: 1979 aa, 228 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC104906
• Gene symbol: MYH11
• Gene ID (NCBI): 4629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Regulated lysosomal exocytosis mediates cancer progression.; Authors - Eda Machado
human	WB	J Clin Invest; UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease.; Authors - Leonardo Elia
mouse	WB	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
rat	WB	Acta Physiol (Oxf); Prostacyclin facilitates vascular smooth muscle cell phenotypic transformation via activating TP receptors when IP receptors are deficient.; Authors - Ziqing Li
rat	WB	Biomed Pharmacother; Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.; Authors - Ji-Ru Zhang
rat	WB	Oxid Med Cell Longev; S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways.; Authors - Moran Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tom (Verified Customer) (07-12-2023)

One of the best smooth muscle cell markers for immunofluorescence!!

• Applications: Immunofluorescence

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C

MYH10 Polyclonal antibody

21403-1-AP

MYLK Polyclonal antibody

21642-1-AP

Beta Actin Monoclonal antibody

60008-1-Ig

TPM3 Polyclonal antibody

55444-1-AP