Myoglobin Monoklonaler Antikörper

Myoglobin Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A4F7

Kat-Nr. : 66205-1-Ig

Synonyme

MB, 1A4F7, EC:1.11.1.-, EC:1.7.-.-, Nitrite reductase MB

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  WB analysis of human skeletal muscle using 66205-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66205-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66205-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 66205-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit heart using 66205-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66205-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66205-1-Ig (Myoglobin antibody at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 66205-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 66205-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66205-1-Ig (Myoglobin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse tongue using 66205-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66205-1-Ig (Myoglobin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of C2C12 using 66205-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myoglobin (66205-1-Ig, Clone:1A4F7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, humanes Herzgewebe, Mausherzgewebe, Hausschwein-Herzgewebe, Kaninchenherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, Mauszungengewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:80000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66205-1-Ig bindet in WB, IHC, FC (Intra), ELISA Myoglobin und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Myoglobin fusion protein Ag8979
• Vollständiger Name: myoglobin
• Berechnetes Molekulargewicht: 154 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17-18 kDa
• GenBank-Zugangsnummer: BC014547
• Gene symbol: Myoglobin
• Gene ID (NCBI): 4151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

Publikationen

Species	Application	Title
pig	WB	Stress Biol; Time-restricted feeding relieves high temperature-induced impairment on meat quality by activating the Nrf2/HO-1 pathway, modification of muscle fiber composition, and enriching the polyunsaturated fatty acids in pigs; Authors - Zhaojian Li