PPP3CA Polyklonaler Antikörper

PPP3CA Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 13422-1-AP

Synonyme

CALNA, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CALN, CALNA1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaus-Skelettmuskelgewebe, Mausherzgewebe, RAW 264.7-Zellen, SH-SY5Y-Zellen
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Zervixkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCSH-SY5Y-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13422-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA PPP3CA und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Affe, Hausschwein, Huhn, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPP3CA fusion protein Ag4203
Vollständiger Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Berechnetes Molekulargewicht 59 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC025714
Gene symbol PPP3CA
Gene ID (NCBI) 5530
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PPP3CA antibody 13422-1-APProtokoll herunterladen
IHC protocol for PPP3CA antibody 13422-1-APProtokoll herunterladenl
IF protocol for PPP3CA antibody 13422-1-APProtokoll herunterladen
IP protocol for PPP3CA antibody 13422-1-APProtokoll herunterladen
FC protocol for PPP3CA antibody 13422-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Mol Psychiatry

A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.

Authors - Yue Qin
mouseWB

J Adv Res

Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling

Authors - Di Fan
mouseWB

Proc Natl Acad Sci U S A

Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.

Authors - Xiju He
pigWB

J Agric Food Chem

Dietary Taurine Supplementation Improves the Meat Quality, Muscle Fiber Type, and Mitochondrial Function of Finishing Pigs

Authors - Wenting An
pigWB

J Cell Physiol

Taurine promotes muscle fiber type transformation through CaN/NFATc1 signaling in porcine myoblasts

Authors - Wenting An
humanWB,IF

Neurobiol Dis

Calcineurin β protects brain after injury by activating the unfolded protein response.

Authors - Yanan Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

nuo (Verified Customer) (08-22-2021)

A very good ab to probe PPP3CA by WB. With a high titer.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain lysate
PPP3CA Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:13422-1-AP)