PKC Gamma Polyklonaler Antikörper

PKC Gamma Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 14364-1-AP

Synonyme

PRKCG, Protein kinase C gamma type, PKC-gamma, PKCG, PKCC

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  WB analysis of mouse brain using 14364-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14364-1-AP (PKC gamma antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14364-1-AP (PKC gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14364-1-AP

  IP result of anti-PKC Gamma (IP:14364-1-AP, 4ug; Detection:14364-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14364-1-AP (PKC gamma antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14364-1-AP (PKC gamma antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cerebellum using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14364-1-AP (PKC gamma antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cerebellum using 14364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14364-1-AP (PKC gamma antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 14364-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 14364-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14364-1-AP (PKC gamma antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 14364-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Gamma (14364-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Cerebellum-Gewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

14364-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA PKC Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Gamma fusion protein Ag5681
• Vollständiger Name: protein kinase C, gamma
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 76-80 kDa
• GenBank-Zugangsnummer: BC047876
• Gene symbol: PKC Gamma
• Gene ID (NCBI): 5582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

Publikationen

Species	Application	Title
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
rat	WB	Chemosphere; Perinatal exposure to nonylphenol impairs dendritic outgrowth of cerebellar Purkinje cells in progeny.; Authors - Mingdan You
human	WB	Cell Oncol (Dordr); The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells; Authors - Yao-Qi Zhou
mouse	IHC	J Neurosci; Dendritic Spine Dynamics after Peripheral Nerve Injury: An Intravital Structural Study.; Authors - Curtis A Benson
rat	WB	J Ethnopharmacol; Analgesic effects and possible mechanisms of iridoid glycosides from Lamiophlomis rotata (Benth.) Kudo in rats with spared nerve injury.; Authors - Yanan Zheng
mouse	WB,IF	Inflamm Bowel Dis; LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin.; Authors - Xiaomin Su

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chiara (Verified Customer) (10-04-2021)

Clear single band

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: mesenchymal Stem cell