RAB10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB10 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27094-1-AP

Synonyme

EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10

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  WB analysis of MDCK using 27094-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27094-1-AP

  WB result of RAB10 antibody (27094-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.

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  WB analysis of HeLa using 27094-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27094-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27094-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 27094-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB10 antibody (27094-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 27094-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RAB10 antibody (27094-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 27094-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RAB10 (27094-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, MDCK-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

27094-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA RAB10 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB10 fusion protein Ag25830
• Vollständiger Name: RAB10, member RAS oncogene family
• Berechnetes Molekulargewicht: 200 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000896
• Gene symbol: RAB10
• Gene ID (NCBI): 10890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.

Publikationen

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
mouse	IF	Biochem Biophys Res Commun; Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation; Authors - Miku Fukawa