Rabbit IgG control Polyklonaler Antikörper

Rabbit IgG control Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

FC (Intra)

Konjugation

FITC Fluorescent Dye

Publikationen(2)

Kat-Nr. : FITC-30000

Synonyme

Rabbit IgG, Rabbit Immunoglobulin, Rabbit IgG isotype, Isotype control, IgG control

---

• 

  FC experiment of HeLa using FITC-30000

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug FITC Anti-Rabbit IgG control (FITC-30000) (red), or 0.4 ug FITC-conjugated ATF4 Rabbit PolyAb (FITC-10835) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

FITC-30000 bindet in FC (Intra) Rabbit IgG control und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: Rabbit IgG control
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publikationen

Species	Application	Title
		Virulence; Exploring the modulatory impact of isosakuranetin on Staphylococcus aureus: Inhibition of sortase A activity and α-haemolysin expression; Authors - Lili Tian
		Nat Commun; Recurrent evolution of adhesive defence systems in amphibians by parallel shifts in gene expression; Authors - Shabnam Zaman