S100B Polyklonaler Antikörper

S100B Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(136)

Rezensionen (3)

Kat-Nr. : 15146-1-AP

Synonyme

S100 beta, Protein S100 B, Protein S100-B, S100 calcium-binding protein B, S-100 protein beta chain

---

• 

  WB analysis using 15146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100B antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100 beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15146-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100 beta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 15146-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15146-1-AP (S100 beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human malignant melanoma using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15146-1-AP (S100B antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15146-1-AP (S100B antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15146-1-AP (S100B antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15146-1-AP (S100B antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15146-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15146-1-AP (S100 Beta antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using S100B antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using S100B antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using S100B antibody (15146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human malignant melanoma using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human malignant melanoma using 15146-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using S100 Beta antibody (15146-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human astrocytes using 15146-1-AP

  Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

• 

  IF Staining of A375 using 15146-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A375 cells using 15146-1-AP (S100 beta antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A375 using 15146-1-AP

  1X10^6 A375 cells were intracellularly stained with 0.5 ug Anti-Human S100 Beta (15146-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A375-Zellen, C6-Zellen, humanes Hodengewebe, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Appendizitis-Gewebe, humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe, humanes malignes Melanomgewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: human astrocytes, A375-Zellen
• Erfolgreiche Detektion in FC (Intra): A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:4000-1:20000
• Immunhistochemie (IHC): IHC : 1:1000-1:6000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 26 publications below
• IHC: See 28 publications below
• IF: See 80 publications below
• ELISA: See 1 publications below

Produktinformation

15146-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA S100B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: S100B fusion protein Ag7440
• Vollständiger Name: S100 calcium binding protein B
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC001766
• Gene symbol: S100 Beta
• Gene ID (NCBI): 6285
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

S100B belongs to the EF-hand calcium binding proteins and is found primarily in astrocytes in the central nervous system (CNS). S100B has a variety of functions, including calcium homeostasis, cell proliferation, differentiation, migration, and survival, as well as neurite outgrowth and regeneration.

 1. What is the molecular weight of S100B?

S100B protein is composed of non-covalently linked homodimers of 11 kDa size.

2. What is the subcellular localization of S100B?

S100B localizes to the nucleus and cytoplasm, associating with intracellular membranes, the centrosomes, microtubules, and type III intermediate filaments (PMID: 19110011). Additionally, it can be released from astrocytes into the extracellular space and can enter the bloodstream.

3. What is the expression pattern of S100B?

S100B is predominantly expressed in astrocytes and maturing oligodendrocytes but is also present in other cell types such as kidney epithelial cells, neural progenitor cells, pituicytes, ependymocytes, chondrocytes, adipocytes, melanocytes, Langerhans cells, dendritic cells, certain lymphocyte subpopulations, skeletal myofibers, myoblasts, and muscle satellite cells (PMID: 19110011). S100B is a commonly used marker of Schwann cells and reactive astrocytes in ICC, IHC, and WB applications.

4. What is the diagnostic use of S100B in the clinic?

S100B is naturally secreted by astrocytes into the extracellular space and low amounts of S100B can pass through the brain-blood barrier and enter the bloodstream. Elevated levels of S100B in the serum are observed in patients with traumatic head injuries, as well as in patients suffering from neurodegenerative diseases (PMID: 30144068). This increase in S100B levels is attributed to the elevated secretion of S100B protein from astrocytes as part of the physiological response to the injury, as well as to the physical damage of astrocytes and increased blood-brain barrier permeability.

Publikationen

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	IHC	Nature; Regulation of intestinal immunity and tissue repair by enteric glia; Authors - Fränze Progatzky
mouse	IHC	Cell; Mechanoreceptor synapses in the brainstem shape the central representation of touch.; Authors - Brendan P Lehnert
human	IF	Sci Bull (Beijing); Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes; Authors - Jiangbing Xiang
mouse	IF	Nat Commun; Alk1 acts in non-endothelial VE-cadherin+ perineurial cells to maintain nerve branching during hair homeostasis; Authors - Gopal Chovatiya
mouse	IF	Neuron; γ-Protocadherins control synapse formation and peripheral branching of touch sensory neurons; Authors - Shan Meltzer

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Clarisse (Verified Customer) (08-08-2022)

This antibody works very well as an astrocyte marker. In the image: in green mouse anti-S100B (1:500 - 66616-1-Ig) and in magenta rabbit anti-S100B (1:500 - 15146-1-AP). The rabbit antibody has a better performance.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human cerebral organoids

S100B Antibody Immunofluorescence validation (1:500 dilution) in Human cerebral organoids (Cat no:15146-1-AP)

FH

Tongcheng (Verified Customer) (09-08-2021)

This antibody works perfect on human astrocytes.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: astrocyte

FH

Sonia (Verified Customer) (10-21-2019)

Works really well on mouse sciatic nerve cross section (cryostat)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: mouse scitic nerve

S100B Antibody Immunofluorescence, validation (1/400 dilution) in mouse scitic nerve (Cat no:15146-1-AP)